Purpose: The purpose of this study was to compare conventional diagnostic culture (CDC) to 16S ribosomal RNA polymerase chain reaction (PCR) analysis for diagnosing bacterial keratitis.
Methods: Samples collected from 100 consecutive patients presenting to the Royal Liverpool University Hospital with bacterial keratitis were processed using CDC and 16S PCR analysis.
Results: The overall detection rate using both methods was 36%. Of these, 72.2% (26/36) were detected by PCR and 63.9% (23/36) isolated by CDC ( = 0.62). Using a combination of both PCR and CDC increased the detection rate for pathogenic bacteria by 13% compared to using CDC alone ( = 0.04). In CDC negative samples, 16S PCR identified more pathogens than CDC in 16S PCR negative samples. Neither order of sample collection nor prior antimicrobial use affected the detection rate.
Conclusions: 16S rRNA gene PCR performed in addition to CDC on corneal samples from patients with clinically suspected bacterial keratitis led to additional pathogen detection.
Translational Relevance: 16S rRNA gene PCR should be developed to become an additional part of clinical service for patients with bacterial keratitis rather than used in isolation.
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http://dx.doi.org/10.1167/tvst.9.13.2 | DOI Listing |
Eye Vis (Lond)
January 2025
ELZA Institute AG, Bahnhofstrasse 15, 8001, Zurich, Switzerland.
Background: Acanthamoeba keratitis (AK) is the most challenging corneal infection to treat, with conventional therapies often proving ineffective. While photoactivated chromophore for keratitis-corneal cross-linking (PACK-CXL) with riboflavin/UV-A has shown success in treating bacterial and fungal keratitis, and PACK-CXL with rose bengal/green light has demonstrated promise in fungal keratitis, neither approach has been shown to effectively eradicate AK. This case study explores a novel combined same-session treatment approach using both riboflavin/UV-A and rose bengal/green light in a single procedure.
View Article and Find Full Text PDFZhonghua Yan Ke Za Zhi
January 2025
Department of Ophthalmology,Beijing Hospital, National Center of Gerontology Institute of Geriatric Medicine,Chinese Academy of Medical Sciences,Beijing100730,China.
Small Methods
December 2024
Institute of Translational Medicine, Nanjing Drum Tower Hospital, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.
Colored contact lenses have gained popularity among young individuals owing to their ability to alter the appearance of the wearer's eyes. However, conventional lenses containing chemical dyes are susceptible to detachment of the pigment layer, which can lead to corneal damage. In this research, a novel cellulose-based structural color contact lens (SCCL) is presented that enhances aesthetic appeal via a cholesteric liquid crystal (CLC) layer.
View Article and Find Full Text PDFOpen Vet J
November 2024
Perth Animal Eye Hospital, Manning, Perth, Western Australia.
Background: Bacterial contamination of medical equipment is a significant risk in both human and veterinary medicine, leading to potential cross-contamination between clinicians, technicians, patients, and healthcare professionals. Pathogens can persist on surfaces, resulting in healthcare-associated infections. Tonometry used to measure intraocular pressure for diagnosing conditions like glaucoma and uveitis, is crucial in both human and veterinary care.
View Article and Find Full Text PDFPol J Vet Sci
June 2024
Siirt University, Faculty of Veterinary Medicine, Department of Surgery, Siirt/Türkiye.
Infectious keratoconjunctivitis is an infectious disease that negatively affects animal welfare causing systemic or local clinical signs in small ruminants and causes significant economic losses in herds. It is important to determine the etiologic agent causing the infection in the development of the protection and control strategies against the disease. The aim of this study was to determine the presence of infectious keratoconjunctivitis cases in small ruminants raised in Siirt province in Türkiye.
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