Optimization of eco-friendly novel amendments for sustainable utilization of Fly ash based on growth performance, hormones, antioxidant, and heavy metal translocation in chickpea (Cicer arietinum L.) plant.

The present study has focused on the evaluation of the maximum amount of flyash (FA) utilization augmentation in the acidic soil (pH 6.1) with the manure during the growth performance and impact of toxic heavy metals under the pot experiment. The 15 days pre-incubated 40% FA treated combination (T16) significantly (P < 0.05) influenced growth performance of chickpea plant after 60 days. The dry weight as well as the contents of N, P, and K increased from 38.8 to 78.53%, 118 to 86%, 148 to 115%, and 95.8 to 95%, respectively, over control in T15 combination after 30 and 60 days. T15 and T16 both treatments induced a significant rise in IAA and GB from 76 to 75.5% and 50%-45%, respectively, after 60 days. The significant increase in the activities of SOD, APX, CAT, and GR with 47%, 56%, 42%, and 28%, respectively, over control was observed in T16 treated combination after 60 days. The significant (P < 0.05) influence in the antioxidant activities, levels of GB, Proline, TSS, and RS were observed across the treatments and durations. The levels of BCF and TF both were <1 in T16 treated plants for toxic heavy metals (Pb, Mo, Cd, and Al), which indicated a negligible extent of translocation from root to shoot and shoot to edible parts in the plants. The results demonstrated that 40% FA supplementation with manure could induce the growth of chickpea in slightly acidic soil and reduce the translocation of toxic metals in the edible parts of the plant.