Colombia has one of the highest rates of sexually transmitted infections (STIs) and teenage pregnancies among Latin American countries. Knowledge about HIV/AIDS and other STIs has been identified as a factor in promoting healthy sexual behavior. The HIV and Other Sexually Transmitted Infections Knowledge Scale (KSI) is an instrument created in Spain to assess sexual health knowledge in adolescents. In view of the lack of scales that measure this construct in Colombia, this study aims to culturally adapt and validate the KSI for the Colombian adolescent population. The sample was comprised of 866 adolescent school children (458 females and 408 males) aged between 14 and 19 years ( = 15.97, = 1.36) from 12 schools in the cities of Bogotá (n = 467) and Barranquilla (n = 400). Results showed good item psychometric properties and adequate discrimination and difficulty indices. Factorial analysis confirmed a five-dimensional factor structure. The indicators of validity showed significant correlations with constructs related on theoretical grounds. In sum, the study presents a valid and reliable scale for evaluating knowledge about HIV and other STIs in Colombian adolescent population.
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