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Objective: Transgenic hens hold a great promise to produce various valuable proteins. Through virus transduction into stage X embryo, the transgene expression under the control of constructed chicken ovalbumin promoters has been successfully achieved. However, a validation system that can evaluate differently developed ovalbumin promoters in in vitro, remains to be developed.
Methods: In the present study, chicken oviduct epithelial cells (cOECs) were isolated from oviduct tissue and shortly cultured with keratinocyte complete medium supplemented with chicken serum. The isolated cells were characterized with immunofluorescence, western blot, and flow cytometry using oviduct-specific marker. Chicken mutated ovalbumin promoter (Mut-4.4-kb-pOV) was validated in these cells using luciferase reporter analysis.
Results: The isolated cOECs revealed that the oviduct-specific marker, ovalbumin protein, was clearly detected by immunofluorescence, western blot, and flow cytometry analysis revealed that approximately 79.40% of the cells contained this protein. Also, luciferase reporter analysis showed that the constructed Mut-4.4-kb-pOV exhibited 7.1-fold (p<0.001) higher activity in the cOECs.
Conclusion: Collectively, these results demonstrate the efficient isolation and characterization of cOECs and validate the activity of the constructed ovalbumin promoter in the cultured cOECs. The in vitro validation of the recombinant promoter activity in cOECs can facilitate the production of efficient transgenic chickens for potential use as bioreactors.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255889 | PMC |
http://dx.doi.org/10.5713/ab.20.0627 | DOI Listing |
Neurogastroenterol Motil
December 2024
Laboratoire Matière et Systèmes Complexes UMR 7057, Université Paris Cité/CNRS, Paris, France.
Background: The gut, the ureter, or the Fallopian tube all transport biological fluids by generating trains of propagating smooth muscle constrictions collectively known as peristalsis. These tubes connect body compartments at different pressures. We extend here Poiseuille's experiments on liquid flow in inert tubes to an active, mechanosensitive tube: the intestine.
View Article and Find Full Text PDFPoult Sci
December 2024
College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, No.6 North Longzihu Road, Zhengdong New District, Zhengzhou, 450046, Henan, China. Electronic address:
Virol J
October 2024
Department of Veterinary Pathology and Public Health, Agronomy and Veterinary Institute Hassan II, Rabat-Instituts, Rabat, BP 6202, Morocco.
Poult Sci
December 2024
Department of Poultry Science, Mississippi State University, MS, 39762. Electronic address:
Anim Genet
December 2024
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan, China.
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