AI Article Synopsis
- MdANK2B is an ankyrin repeat-containing protein in apples (Malus domestica) that enhances resistance to salt stress and reduces sensitivity to the plant hormone abscisic acid (ABA).
- The protein is located in the cytoplasm and its gene is highly expressed in apple leaves and fruits, showing increased activity under salt stress and ABA conditions.
- Overexpressing MdANK2B in apple plants leads to less accumulation of reactive oxygen species (ROS) during salt stress by boosting antioxidant enzyme activity, highlighting its important role in helping the plant cope with environmental stress.
Article Abstract
The ankyrin repeat-containing protein MdANK2B was identified to contribute to increasing resistance to salt stress and decreasing sensitivity to ABA in Malus domestica. Ankyrin (ANK) repeat-containing proteins occur widely in prokaryotes, eukaryotes, and even in some viruses and play a critical role in plant growth and development, as well as the response to biotic and abiotic stress. However, the function of ANK repeat-containing proteins in apple (Malus domestica) has not yet been investigated. Here, we identified apple MdANK2B based on homology analysis with the Arabidopsis ANK repeat-containing proteins AtAKR2A and AtAKR2B. MdANK2B was found to be localized in the cytoplasm, and its encoding gene was highly expressed in both apple leaves and fruits. In addition, MdANK2B gene expression was highly induced by salt stresses and abscisic acid (ABA). Overexpression of MdANK2B increased resistance to salt stress and decreased sensitivity to ABA in both transgenic apple calli and seedlings. In addition, overexpression of MdANK2B reduced the accumulation of reactive oxygen species (ROS) by enhancing the activity of antioxidant enzymes in response to salt stress. Our data revealed the role of MdANK2B in response to salt stress and ABA treatment in apple, which widens the known functions of ANK repeat-containing proteins in response to abiotic stress.
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| http://dx.doi.org/10.1007/s00299-020-02642-9 | DOI Listing |
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