Molecular Mechanism of the UDP-Glucuronosyltransferase 2B20-like Gene () in Pesticide Resistance of .

UDP-glucuronosyltransferases (UGTs), being multifunctional detoxification enzymes, play a major role in the process of resistance to various pesticides in insects. However, the mechanism underlying the molecular regulation of pesticide resistance remains unclear, especially in . In this study, all of the UGTs in (AccUGT) have been identified through the multiple alignment and phylogenetic analysis. Expression of AccUGT genes under different pesticides, and antioxidant genes after silencing of , were detected by qRT-PCR. The resistance of overexpressed to oxidative stress was investigated by an overexpression system. Also, antioxidant-related enzyme activity was detected after silencing of the gene. Expression pattern analysis showed that almost all UGT genes were upregulated under different pesticide treatments. This result indicated that AccUGTs participate in the detoxification process of pesticides. was the major gene because it was more highly induced than the others. Overexpression of in could effectively improve oxidative stress resistance. Specifically, silencing the gene increased oxidative stress by repressing the expression of oxidation-related genes, decreasing antioxidant-related enzyme activity, and increasing malondialdehyde concentration. Taken together, our results indicate that AccUGTs are involved in pesticide resistance, among which, contributes to the detoxification of pesticides by eliminating oxidative stress in . This study explains the molecular basis for the resistance of bees to pesticides and provides an important safeguard for maintaining ecological balance.