AI Article Synopsis
- Long noncoding RNAs (lncRNAs) play a significant role in regulating DNA, RNA, and protein interactions but direct binding to DNA is not commonly shown.
- This text outlines computational strategies for identifying lncRNAs that can bind to chromatin through stable DNA-DNA-RNA triplex formations.
- Additionally, it highlights biophysical methods for experimentally validating the lncRNA-target gene interactions selected through computational means.
Article Abstract
Long noncoding RNAs are well studied for their regulatory actions through interaction with DNA regulating biological roles of DNA, RNA, or protein. However, direct binding of lncRNA with DNA is rarely demonstrated in experiments. The present protocol explains genome wide computational strategies to choose lncRNAs that can bind directly to the chromatin by forming highly stable DNA-DNA-RNA triplexes. The chapter also focuses on biophysical methods that can be used to validate the computationally derived lncRNA-gene targets in vitro.
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| http://dx.doi.org/10.1007/978-1-0716-1158-6_5 | DOI Listing |
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