Hyperspectral imaging provides spatially resolved spectral information. Utilizing dual-frequency combs as active illumination sources, hyperspectral imaging with ultra-high spectral resolution can be implemented in a scan-free manner when a detector array is used for heterodyne detection. Here, we show that dual-comb hyperspectral imaging can be performed with an uncooled near-to-mid-infrared detector by exploiting the detector array's high frame rate, achieving 10 Hz acquisition in 30 spectral channels across 16,384 pixels. Artificial intelligence (AI) enables real-time data reduction and imaging of gas concentration based on characteristic molecular absorption signatures. Owing to the detector array's sensitivity from 1 to 5 µm wavelength, this demonstration lays the foundation for real-time versatile imaging of molecular fingerprint signatures across the infrared wavelength regime with high temporal resolution.
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http://dx.doi.org/10.1364/OL.410762 | DOI Listing |
Chem Biomed Imaging
January 2025
College of Biomedical Engineering & Instrument Science, Key Laboratory for Biomedical Engineering of Ministry of Education, Zhejiang University, Hangzhou 310058, China.
Studying embryogenesis is fundamental to understanding developmental biology and reproductive medicine. Its process requires precise spatiotemporal regulations in which lipid metabolism plays a crucial role. However, the spatial dynamics of lipid species at the subcellular level remains obscure due to technical limitations.
View Article and Find Full Text PDFChem Biomed Imaging
January 2025
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, Indiana 47907, United States.
Due to uncontrolled cell proliferation and disrupted vascularization, many cancer cells in solid tumors have limited oxygen supply. The hypoxic microenvironments of tumors lead to metabolic reprogramming of cancer cells, contributing to therapy resistance and metastasis. To identify better targets for the effective removal of hypoxia-adaptive cancer cells, it is crucial to understand how cancer cells alter their metabolism in hypoxic conditions.
View Article and Find Full Text PDFJ Pharm Biomed Anal
January 2025
Institute of Chemistry, University of Silesia in Katowice, 9 Szkolna Street, Katowice 40-006, Poland; SPIN-Lab Centre for Microscopic Studies on Matter, University of Silesia in Katowice, 75 Pulku Piechoty Street 1, Chorzow 41-500, Poland. Electronic address:
Near-infrared hyperspectral imaging (NIR-HSI) integrated with expert systems can support the monitoring of active pharmaceutical ingredients (APIs) and provide effective quality control of tablet formulations. However, existing quality control methods usually test a limited number of variability sources affecting the final product. This study examines the potential of NIR-HSI (in the spectral range of 935.
View Article and Find Full Text PDFAnal Chem
January 2025
Nanophotonic Systems Laboratory, Department of Mechanical and Process Engineering, ETH Zurich, 8092 Zurich, Switzerland.
Droplet-based microfluidics is a powerful tool for high-throughput analysis of liquid samples with significant applications in biomedicine and biochemistry. Nevertheless, extracting content-rich information from single picolitre-sized droplets at high throughputs remains challenging due to the weak signals associated with these small volumes. Overcoming this limitation would be transformative for fields that rely on high-throughput screening, enabling broader multiparametric analysis.
View Article and Find Full Text PDFJ R Soc Interface
January 2025
Faculty of Information Technology, University of Jyväskylä, Jyvaskyla, Finland.
The design of photobioreactors for microalgae cultivation aims to achieve an architecture that allows the most efficient photosynthetic growth. The availability of light at wavelengths that are important for photosynthesis is therefore particularly crucial for reactor design. While testing different reactor types in practice is expensive, simulations could effectively limit the range of material and reactor design options.
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