Long Non-coding RNA Regulates Porcine Satellite Cell Differentiation Through / and .

The gene promotes skeletal muscle differentiation in mice, but the regulatory models and mechanisms of myogenesis regulated by are largely unknown in pigs. Therefore, the regulatory modes of in the differentiation of porcine skeletal muscle satellite cells (PSCs) need to be determined. We observed that gene silencing could decrease the expressions of the myogenin () gene, myogenic differentiation (), and myosin heavy chain () in PSCs. Therefore, we constructed and sequenced 12 cDNA libraries of PSCs after knockdown of at two differentiation time points to analyze the transcriptome differences. A total of 11,419 differentially expressed genes (DEGs) were identified. Among these DEGs, we found through bioinformatics analysis and protein interaction experiment that SRY-box transcription factor 4 () and Drebrin 1 () were the key genes in -regulated PSC differentiation. Functional analysis shows that and promote PSC differentiation. Mechanistically, regulates PSC differentiation through two different pathways. On the one hand, functions as a molecular sponge of , which inhibits the differentiation of PSCs, thereby modulating the derepression of . On the other hand, regulates PSC differentiation through directly binding with DBN1. Furthermore, binds to the promoters of and . The knockdown of inhibits the expression of and . We determined the function of and provided a molecular model to elucidate 's role in regulating PSC differentiation.