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Identification and Characterization of a New Type III Polyketide Synthase from a Marine Yeast, . | LitMetric

AI Article Synopsis

  • - A new Type III Polyketide synthase (PKSIII) gene from a marine yeast strain Mo29 was discovered, which is distinct from known terrestrial fungal sequences due to a unique 74-amino acid extension at the C-terminus.
  • - The full-length and truncated versions of this PKSIII were expressed in E. coli BL21 (DE3), revealing that the extra C-terminal amino acids don’t affect its biochemical functions, as both versions exhibited the same activity.
  • - Analysis showed that the recombinant PKSIII can produce tri- and tetraketide pyrones and alkylresorcinols using specific fatty acid chains, with some of these compounds demonstrating cytotoxic effects against various cancer cell

Article Abstract

A putative Type III Polyketide synthase (PKSIII) encoding gene was identified from a marine yeast, strain Mo29 (UBOCC-A-208024) (formerly named as sp.) isolated from deep-sea hydrothermal vents. This gene is part of a distinct phylogenetic branch compared to all known terrestrial fungal sequences. This new gene encodes a C-terminus extension of 74 amino acids compared to other known PKSIII proteins like . Full-length and reduced versions of this PKSIII were successfully cloned and overexpressed in a bacterial host, BL21 (DE3). Both proteins showed the same activity, suggesting that additional amino acid residues at the C-terminus are probably not required for biochemical functions. We demonstrated by LC-ESI-MS/MS that these two recombinant PKSIII proteins could only produce tri- and tetraketide pyrones and alkylresorcinols using only long fatty acid chain from C8 to C16 acyl-CoAs as starter units, in presence of malonyl-CoA. In addition, we showed that some of these molecules exhibit cytotoxic activities against several cancer cell lines.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763939PMC
http://dx.doi.org/10.3390/md18120637DOI Listing

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