Purpose: Pneumonia is a respiratory disease with an increasing incidence in recent years. More and more studies have revealed that lncRNAs can regulate the transcriptional expression of target genes at different stage. Herein, we aimed to explore the effect of lncRNA MIAT in LPS-induced pneumonia, and further illuminate the possible underlying mechanisms.
Method And Results: Mice were intraperitoneally injected with LPS, and the lung inflammation was evaluated. Microarray showed lncRNA MIAT was up-regulated in LPS-induced pulmonary inflammation. And qRT-PCR and FISH assay indicated that MIAT was increased in mice with LPS injection. Functional analysis showed sh-MIAT inhibited LPS-induced inflammation response, inhibited apoptosis level and protected lung function. As well, si-MIAT removed the injury of LPS on mouse lung epithelial TC-1 cells, and inhibited the activation of NF-κB signaling. Furthermore, MIAT acted as a sponge of miR-147a, and miR-147a directly targeted NKAP. Functionally, AMO-147a or NKAP remitted the beneficial effects of si-MIAT on LPS-induced inflammation response of TC-1 cells.
Conclusion: Deletion of MIAT protected against LPS-induced lung inflammation via regulating miR-147a/NKAP, which might provide new insight for pneumonia treatment.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880384 | PMC |
http://dx.doi.org/10.18632/aging.202284 | DOI Listing |
Data Brief
December 2024
GenPhySE, Université de Toulouse, INRAE, INPT, ENVT, F31326 Castanet Tolosan, France.
Limiting the level of piglet losses before weaning is a growing demand from producers and society to improve the welfare and health of sows and piglets. In particular, perinatal mortality, which can be defined as the complete development allowing survival at birth, is mostly due to reduced piglet maturity that occurs at the end of gestation. Fetal growth and maturation depend on a fine balance between the nutrient requirements for optimal fetal growth and the maternal nutrient requirements.
View Article and Find Full Text PDFClin Neurol Neurosurg
November 2024
Department of Critical Care Medicine, The First People's Hospital of Ziyang, Ziyang 641300, China. Electronic address:
Objective: This study aims to explore the clinical significance of long non-coding RNA, myocardial infarction-associated transcript (MIAT), in patients with traumatic brain injury (TBI).
Methods: Retrospective inclusion of TBI patients meeting clinical criteria with complete data, alongside healthy controls. RT-qPCR was used to detect the expression of the serum MIAT.
Int J Biol Macromol
December 2024
Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, China. Electronic address:
Bronchopulmonary dysplasia (BPD) manifests in premature neonates with aberrant pulmonary function. Numerous long non-coding RNAs (lncRNAs) have been implicated in the pathogenesis of BPD. This study aims to elucidate the impact of the lncRNA myocardial infarction-associated transcript (MIAT) on the initiation and progression of BPD.
View Article and Find Full Text PDFDiabetes Metab Syndr Obes
November 2024
Ophthalmology Center, Suining Central Hospital, Suining, People's Republic of China.
Int J Mol Sci
October 2024
School of Physical Science and Technology, Inner Mongolia University, Hohhot 010021, China.
Aberrant DNA methylation plays a crucial role in breast cancer progression by regulating gene expression. However, the regulatory pattern of DNA methylation in long noncoding RNAs (lncRNAs) for breast cancer remains unclear. In this study, we integrated gene expression, DNA methylation, and clinical data from breast cancer patients included in The Cancer Genome Atlas (TCGA) database.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!