AI Article Synopsis
- Variation in DNA shape is key for regulating gene expression and interactions with drugs, but tracking these transient changes is difficult.
- A new label-free fluorescence technique using the DNA intercalator K21 can monitor these conformational changes by detecting the ratio of monomer to excimer emissions.
- This method allows for the analysis of DNA shape dynamics in various structures, offering insights into transient genomic processes in living cells, as demonstrated through studies on plasmid DNA and enzyme digestion.
Article Abstract
Variation of DNA conformation is important in regulating gene expression and mediating drug-DNA interactions. However, directly probing transient DNA conformation changes is challenging owing to the dynamic nature of this process. We show a label-free fluorescence method to monitor transient DNA conformation changes in DNA structures with various lengths and shapes using a DNA intercalator, K21. K21 can form transient excimers on the surface of DNA; the ratiometric emission of monomer and excimer correlate to DNA transient conformation stability in numerous DNA structures, including i-motifs, G-quadruplex structures, and single nucleotide mutation at random position. We analyzed the conformation dynamics of a single plasmid before and after enzyme digestion with confocal fluorescence microscopy. This method provides a label-free fluorescence strategy to probe transient conformation changes of DNA structures and has potential in uncovering transient genomic processes in living cells.
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| http://dx.doi.org/10.1002/anie.202014466 | DOI Listing |
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