This study employed mesophilic Bacillus subtilis RTS strain isolated from soil with high xylanolytic activity. A 642 bp (xyn) xylanase gene (GenBank accession number MT677937) was extracted from Bacillus subtilis RTS and cloned in Escherichia coli BL21 cells using pET21c expression system. The cloned gene belongs to glycoside hydrolase family 11 with protein size of approximately 23 KDa. The recombinant xylanase showed optimal enzyme activity at 60 °C and at pH 6.5. Thermostability of recombinant xylanase was observed between the temperature range of 30-60 °C. Xylanase also remained stable in different concentration of various organic solvents (ethanol, butanol). This might be due to the formation of protein/organic solvent interface which prevents stripping of essential water molecules from enzyme, thus enzyme conformation and activity remained stable. Finally, the molecular docking analysis through AutoDock Vina showed the involvement of Tyr 108, Arg140 and Pro144 in protein-ligand interaction, which stabilizes this complex. The observed stability of recombinant xylanase at higher temperature and in the presence of organic solvent (ethanol, butanol) suggested possible application of this enzyme in biofuel and other industrial applications.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijbiomac.2020.12.001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Therapeutic potential of Bacillus-derived lipopeptides in controlling enteropathogens and modulating immune responses to mitigate post-weaning diarrhea in swine.
Vet Res Commun
January 2025
Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta N 36 Km 601, Río Cuarto City, 5800, Córdoba, Argentina.
Post-weaning diarrhea (PWD) is a major concern for pig producers, as stress and early weaning increase susceptibility to enteropathogens like enterotoxigenic Escherichia coli (ETEC) and Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium).
View Article and Find Full Text PDFEnhanced pullulanase production through expression system optimization and biofilm-immobilized fermentation strategies.
Int J Biol Macromol
January 2025
National Engineering Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, PR China; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, PR China; Soochow University, Suzhou, Jiangsu 215123, PR China.
Pullulanase (PUL) plays a crucial role in breaking down α-1,6-glycosidic bonds in starch, a key process in starch processing and conversion. Based on PulB with high enzymatic activity, the expression of PUL in Bacillus subtilis was enhanced by plasmid screening, double promoter optimization, and signal peptide engineering. Furthermore, we innovatively employed a mussel foot protein to enhance the cell adhesion to carriers and utilized biofilm-based cell immobilization technology to optimize the fermentation process and stimulate biofilm formation.
View Article and Find Full Text PDFMarine microbes suppressed Vibrio and enhanced biological performance of euryhaline rotifer, Brachionus plicatilis.
Mar Pollut Bull
January 2025
Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266100, China. Electronic address:
The excessive use of antibiotics in mariculture has surpassed permitted levels, leading to their release into surrounding waters and accumulation in cultured organisms, which poses risks to human health and highlighting the urgent need for alternatives to reduce antibiotic use. Therefore, the present study aimed to test four microbes including Debaryomyces hansenii, Ruegeria mobilis, Lactobacillus plantarum and Bacillus subtilis, on lowering Vibrio, promoting population increase and survival of Brachionus plicatilis. The digestive enzymes activity including α-amylase, lipase and protease, microbial retention and biochemical composition of rotifers were analyzed.
View Article and Find Full Text PDFUsing environment-sensitive tetramethylated thiophene-BODIPY fluorophores in DNA probes for studying effector-induced conformational changes of protein-DNA complexes.
RSC Chem Biol
January 2025
Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences Flemingovo n. 2 Prague 6 Czechia
The LutR protein represses the transcription of genes encoding enzymes for the utilization of l-lactate in through binding to a specific DNA region. In this study, we employed oligonucleotide probes modified by viscosity-sensitive tetramethylated thiophene-BODIPY fluorophores to investigate the impact of selected metabolites on the LutR-DNA complex. Our goal was to identify the effector molecule whose binding alters the protein-DNA affinity, thereby enabling gene transcription.
View Article and Find Full Text PDFCombined application of methyl jasmonate and Bacillus subtilis LLCG89 suppresses blue and green mold of lemon (Citrus limon L.).
Pest Manag Sci
January 2025
Department of Plant Pathology, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Background: Bacillus species produce antimicrobial lipopeptides (LPs) and methyl jasmonate (MeJA) induces resistance in harvested fruits against postharvest pathogens. However, there is limited evidence of the combined efficacy of Bacillus LPs and MeJA to suppress postharvest diseases.
Results: This study presents the combined effect of Bacillus LPs and MeJA to suppress P.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!