AI Article Synopsis
- A new transformation assay has been developed to detect oncogenes in DNA from human tumor cell lines, using NIH3T3 murine fibroblasts for transfection.
- Out of 17 human tumor cell lines tested, 8 produced transformed cells, indicating the presence of oncogenes.
- Three transforming activities are linked to activated ras genes, while an osteosarcoma cell line (SAOS2) showed an activated oncogene not identified among 18 known ones, and another oncogene was found due to an unexpected DNA rearrangement.
Article Abstract
Oncogenes in DNAs from human tumor cell lines have been detected by a new transformation assay. Cellular DNAs are transfected into NIH3T3 murine fibroblasts, and transformed cells are selected by maintaining cell cultures in a defined medium lacking platelet-derived or fibroblast growth factors. DNAs from eight of 17 human tumor cell lines have yielded transformants by this method. Activated cellular ras genes account for three of the transforming activities. The SAOS2 osteosarcoma cell line contains an activated oncogene distinct from 18 known oncogenes. Another cellular oncogene was detected as the consequence of a fortuitous transfection-mediated DNA rearrangement.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!