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Developing an advanced gut on chip model enabling the study of epithelial cell/fibroblast interactions. | LitMetric

AI Article Synopsis

  • Organoids are useful for studying gut diseases but don't fully replicate the intestinal wall's complexity; a new gut-on-chip model aims to address this issue.
  • This model features a 3D collagen scaffold that mimics the mouse gut's structure, with a laminin coating and a cytocompatible treatment to support primary intestinal cells.
  • The device allows for the development of a polarized epithelial layer and maintains long-term culture under fluid shear stress, providing a better tool for researching gut pathophysiology.

Article Abstract

Organoids are widely used as a model system to study gut pathophysiology; however, they fail to fully reproduce the complex, multi-component structure of the intestinal wall. We present here a new gut on chip model that allows the co-culture of primary epithelial and stromal cells. The device has the topography and dimensions of the mouse gut and is based on a 3D collagen I scaffold. The scaffold is coated with a thin layer of laminin to mimic the basement membrane. To maintain the scaffold structure while preserving its cytocompatibility, the collagen scaffold was rigidified by threose-based post-polymerization treatment. This treatment being cytocompatible enabled the incorporation of primary intestinal fibroblasts inside the scaffold, reproducing the gut stromal compartment. We observed that mouse organoids, when deposited into crypts, opened up and epithelialized the scaffold, generating a polarized epithelial monolayer. Proper segregation of dividing and differentiated cells along the crypt-villus axis was achieved under these conditions. Finally, we show that the application of fluid shear stress allows the long-term culture of this intestinal epithelium. Our device represents a new biomimetic tool that captures key features of the gut complexity and could be used to study gut pathophysiology.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930731PMC
http://dx.doi.org/10.1039/d0lc00672fDOI Listing

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