PET-MRI nanoparticles imaging of blood-brain barrier damage and modulation after stroke reperfusion.

In an acute ischaemic stroke, understanding the dynamics of blood-brain barrier injury is of particular importance for the prevention of symptomatic haemorrhagic transformation. However, the available techniques assessing blood-brain barrier permeability are not quantitative and are little used in the context of acute reperfusion therapy. Nanoparticles cross the healthy or impaired blood-brain barrier through combined passive and active processes. Imaging and quantifying their transfer rate could better characterize blood-brain barrier damage and refine the delivery of neuroprotective agents. We previously developed an original endovascular stroke model of acute ischaemic stroke treated by mechanical thrombectomy followed by positron emission tomography-magnetic resonance imaging. Cerebral capillary permeability was quantified for two molecule sizes: small clinical gadolinium Gd-DOTA (<1 nm) and AGuIX nanoparticles (∼5 nm) used for brain theranostics. On dynamic contrast-enhanced magnetic resonance imaging, the baseline transfer constant was 0.94 [0.48, 1.72] and 0.16 [0.08, 0.33] ×10min, respectively, in the normal brain parenchyma, consistent with their respective sizes, and 1.90 [1.23, 3.95] and 2.86 [1.39, 4.52] ×10min in choroid plexus, confirming higher permeability than brain parenchyma. At early reperfusion, for both Gd-DOTA and AGuIX nanoparticles was significantly higher within the ischaemic area compared to the contralateral hemisphere; 2.23 [1.17, 4.13] and 0.82 [0.46, 1.87] ×10min for Gd-DOTA and AGuIX nanoparticles, respectively. With AGuIX nanoparticles, also increased within the ischaemic growth areas, suggesting added value for AGuIX. Finally, was significantly lower in both the lesion and the choroid plexus in a drug-treated group (ciclosporin A,  = 7) compared to placebo ( = 5). quantification with AGuIX nanoparticles can monitor early blood-brain barrier damage and treatment effect in ischaemic stroke after reperfusion.