Keratinolytic fungi representing the genus that were isolated from the soils of a rook () colony were used as biological agents for the disposal of waste feathers. The aim of this study was to assess the abilities of and fungi with a varied inflow of keratin matter to biodegrade waste feathers. The evaluation was based on the determination of feather mass loss, the activity of keratinolytic enzymes, and the content of mineral N and S forms. It was found that the activity of protease released by the fungi contributed to an increase in the level of soluble proteins and peptides and the concentration of ammonium ions, as well as alkalization of the culture medium. Keratinase activity was significantly correlated with sulfate release, especially in cultures. The strains of fungi isolated from the soil with the highest supply of organic matter, i.e., strains III, IV, and V, had the lowest enzymatic activity, compared to the strains, but they released mineral nitrogen and sulfur forms that are highly important for fertilization, as well as nutritionally important peptides and amino acids. strains can be used for the management of waste feathers that can be applied in agricultural practice.
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http://dx.doi.org/10.3390/ijerph17249162 | DOI Listing |
Food Chem
December 2024
Department of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, Gdańsk, Narutowicza Street 11/12, 80-233, Poland. Electronic address:
This study compared the effectiveness of urea-containing and urea-free L-cysteine solutions in extracting high-quality feather keratin and evaluated commercial proteases for producing keratin-derived bioactive peptides. The urea-assisted extraction was crucial for achieving high structural integrity and yield of soluble keratin. The keratin isolate exhibited oil-holding capacity of 9.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Biomaterials Department, Institute of Industrial Nanomaterials, Kumamoto University, Kumamoto 860-8555, Japan. Electronic address:
The global rise of bacterial resistance demands innovative strategies to enhance antibiotic efficacy. This study investigates keratin nanoparticles (KNPs) derived from waste chicken feathers as sustainable drug carriers. Antibacterial activity of KNPs was evaluated against Staphylococcus aureus and Escherichia coli using antibacterial sensitivity assays, including disc diffusion and minimum inhibitory concentration tests, while cytotoxicity was evaluated on human lymphoma cells.
View Article and Find Full Text PDFFood Chem
January 2025
Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, 25240, Erzurum, Turkey. Electronic address:
Recycling of protein-rich environmental wastes and obtaining more valuable products from these recycled products is a topic of interest for researchers. This study aims to produce, purify, and characterize the physicochemical and structural properties of the protease enzyme produced from Brevibacillus agri SAR25 using salmon fish waste as substrate and also to evaluate the effect of protease on the chicken feather, enzyme-ligand interactions, and active site surface area. The production of protease was optimum on 50 g/L fish waste, pH 8, 40 °C, 96 h, and 150 rpm.
View Article and Find Full Text PDFMolecules
December 2024
Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China.
Discarded sports waste faces bottlenecks in application due to inadequate disposal measures, and there is often a neglect of enhancing resource utilization efficiency and minimizing environmental impact. In this study, nanoporous biochar was prepared through co-hydrothermal carbonization (co-HTC) and pyrolytic activation by using mixed goose feathers and heavy-metals-contaminated pine sawdust. Comprehensive characterization demonstrated that the prepared M-3-25 (Biochar derived from mixed feedstocks (25 mg/g Cu in pine sawdust) at 700 °C with activator ratios of 3) possesses a high specific surface area 2501.
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