AI Article Synopsis
- Conventional histology involves analyzing 2D slices of biopsies, which can be destructive and limit information from 3D structures.
- 3D X-ray histology offers a solution but faces challenges due to soft tissue's low X-ray contrast.
- Staining tissue with brominated fluorescein salts improves contrast, with dibromo fluorescein barium being the most effective agent for 3D imaging and further histological analysis.
Article Abstract
Conventional histology is a destructive technique based on the evaluation of 2D slices of a 3D biopsy. By using 3D X-ray histology these obstacles can be overcome, but their application is still restricted due to the inherently low attenuation properties of soft tissue. In order to solve this problem, the tissue can be stained before X-ray computed tomography imaging (CT) to enhance the soft tissue X-ray contrast. Evaluation of brominated fluorescein salts revealed a mutual influence of the number of bromine atoms and the cations applied on the achieved contrast enhancement. The dibromo fluorescein barium salt turned out to be the ideal X-ray contrast agent, allowing for 3D imaging and subsequent complementing counterstaining applying standard histological techniques.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986394 | PMC |
| http://dx.doi.org/10.1002/chem.202005203 | DOI Listing |
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