Blastomycosis due to and is a significant cause of respiratory mycoses in North America with occasional reported outbreaks. We developed a highly sensitive, specific, and reproducible TaqMan duplex real-time PCR assay for the differentiation of and The new assay permitted retrospective analysis of cultures (2005 to 2019) and primary clinical specimens from blastomycosis cases (2013 to 2019) from New York patients. We identified as the predominant pathogen in 38 cases of blastomycosis, while was a minor pathogen involved in five cases; these findings expand understanding of blastomycosis in New York. The duplex real-time PCR assay could be implemented in reference and public health laboratories to further understand the ecology and epidemiology of blastomycosis due to and .
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| http://dx.doi.org/10.1128/JCM.02078-20 | DOI Listing |
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