Identification by Comprehensive Bioinformatics Analysis of KIF15 as a Candidate Risk Gene for Triple-Negative Breast Cancer.

Cancer Manag Res

Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.

Published: December 2020

Background: Previous studies have shown that kinesin family proteins () play an indispensable roles in several types of cancer. However, the expression and clinical significance of in triple-negative breast cancer remain unclear.

Methods: In this study, the role of , including gene expression analysis, methylation characteristic, CNV characteristic, and miRNA target regulation, was evaluated using multiple bioinformatic tools based on TCGA database. Quantitative real-time PCR and Western blot were used to determine the expression level of in triple-negative breast cancer cell lines. Then, functional experiments were employed to explore the effects of on tumor growth and metastasis in triple-negative breast cancer.

Results: Our data showed that was significantly upregulated in triple-negative breast cancer (TNBC). Functionally, downregulation of significantly facilitated apoptosis and G2/M phase arrest, and inhibited the migration and invasion of TNBC cells. The mechanism of action of was closely related to DNA replication checkpoint and cell cycle regulation in TNBC based on GSEA. In addition, bioinformatics analysis demonstrated that high expression of in TNBC was correlated with copy number aberration and DNA methylation levels.

Conclusion: Our findings suggest that is a novel oncogene in TNBC and provide us a strong evidence that it might be served as a potential clinical target and biomarker in triple-negative breast cancer.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718892PMC
http://dx.doi.org/10.2147/CMAR.S262017DOI Listing

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