AI Article Synopsis
- YangXinDingJi (YXDJ) capsules, derived from traditional Chinese medicine, are investigated for their potential to protect heart function in rats suffering from myocardial ischemia induced by isoproterenol (ISO).
- YXDJ treatment showed significant improvement in heart morphology, reduced oxidative stress, and inflammation, as well as improved L-type calcium channel function.
- The study suggests that YXDJ could be beneficial for treating cardiovascular diseases through its anti-inflammatory and antioxidative properties, but the precise mechanisms behind these effects need further exploration.
Article Abstract
Background: YangXinDingJi (YXDJ) capsule is one of traditional Chinese medicines (TCMs) derived from Zhigancao decoction, which is usually used for the treatment of cardiovascular disease in China. . Cardiovascular events are one of the leading causes of death worldwide. Myocardial ischemia (MI) severely reduces myocyte longevity and function. The YangXinDingJi (YXDJ) capsule has been used in the treatment of clinical cardiac disease in China. Nevertheless, the underlying cellular mechanisms for the benefits to heart function resulting from the use of this capsule are still unclear. The aim of this study was to evaluate the protective effects of the YXDJ on isoprenaline-induced MI in rats and to clarify its underlying myocardial protective mechanisms based on L-type calcium channels and myocardial contractility.
Materials And Methods: Rats were randomly divided into five groups with ten rats in each group: (1) control; (2) ISO-induced model; (3) high-dose YXDJ (2.8 g/kg/day intraperitoneally for five days), (4) low-dose YXDJ (1.4 g/kg/day for five days); and (5) verapamil ( = 10 in each group). Isoproterenol (ISO) was injected subcutaneously for two consecutive days to induce the rat model of MI. Heart and biochemical parameters were obtained. The patch-clamp technique was used to observe the regulatory effects of YXDJ on the L-type calcium current (I) in isolated cardiomyocytes. An IonOptix MyoCam detection system was used to observe the contractility of YXDJ on isolated cardiomyocytes.
Results: YXDJ caused a significant improvement in pathological heart morphology and alleviated oxidative stress and inflammatory responses. Exposure to YXDJ caused a decrease in blockade of I in a concentration-dependent manner.
Conclusions: The results indicate that YXDJ significantly inhibited inflammatory cytokine expressions, oxidative stress, and L-type Ca channels, and decreased contractility in isolated rat cardiomyocytes. These findings may be relevant to the cardioprotective efficacy of YXDJ.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685838 | PMC |
| http://dx.doi.org/10.1155/2020/8539148 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Systematic investigation for the mechanisms and the substance basis of Yang-Xin-Ding-Ji capsule based on the metabolite profile and network pharmacology.
Biomed Chromatogr
December 2021
Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, People's Republic of China.
Because traditional Chinese medicine (TCM) is a complex mixture of multiple components, the application of methodologies for evaluating single-component Western medicine in TCM studies may have certain limitations. Appropriate strategies that recognize the integrality of TCM and connect to TCM theories remain to be developed. Yang-Xin-Ding-Ji (YXDJ) capsule is originally from a classical TCM formula used for the treatment of arrhythmia.
View Article and Find Full Text PDFMechanisms Underlying the Cardioprotection of YangXinDingJi Capsule against Myocardial Ischemia in Rats.
Evid Based Complement Alternat Med
November 2020
School of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China.
Article Synopsis
- YangXinDingJi (YXDJ) capsules, derived from traditional Chinese medicine, are investigated for their potential to protect heart function in rats suffering from myocardial ischemia induced by isoproterenol (ISO).
- YXDJ treatment showed significant improvement in heart morphology, reduced oxidative stress, and inflammation, as well as improved L-type calcium channel function.
- The study suggests that YXDJ could be beneficial for treating cardiovascular diseases through its anti-inflammatory and antioxidative properties, but the precise mechanisms behind these effects need further exploration.
LiaR-independent pathways to daptomycin resistance in Enterococcus faecalis reveal a multilayer defense against cell envelope antibiotics.
Mol Microbiol
March 2019
Center for Antimicrobial Resistance and Microbial Genomics, University of Texas Health Science Center, McGovern School of Medicine, Houston, TX, USA.
The lipopeptide antibiotic daptomycin (DAP) is a key drug against serious enterococcal infections, but the emergence of resistance in the clinical setting is a major concern. The LiaFSR system plays a prominent role in the development of DAP resistance (DAP-R) in enterococci, and blocking this stress response system has been proposed as a novel therapeutic strategy. In this work, we identify LiaR-independent pathways in Enterococcus faecalis that regulate cell membrane adaptation in response to antibiotics.
View Article and Find Full Text PDFCharacterization of the Bacillus subtilis YxdJ response regulator as the inducer of expression for the cognate ABC transporter YxdLM.
Microbiology (Reading)
August 2004
Laboratoire de Chimie Bactérienne, Institut de Biologie Structurale et Microbiologie, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
The genome of Bacillus subtilis, like those of some other AT-rich Gram-positive bacteria, has the uncommon feature of containing several copies of arrangements in which the genes encoding two-component and cognate ABC transporter systems are adjacent. As the function of one of these systems, the product of the yxd locus, is still unknown, it was analysed further in order to get some clues on the physiological role of the gene products it encodes. The yxdJ gene was shown to encode a DNA-binding protein that directly controls transcription of the neighbouring operon encoding the ABC transporter YxdLM.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!