The Allelic Diversity of the Gibberellin Signaling Pathway Genes in Coss.

Gibberellin-insensitive reduced height genes are widely spread in modern wheat varieties, making them resistant to lodging under conditions of intensive farming. However, the limited diversity of these genes present in wheat germplasm can limit the adaptability of newly created cultivars to the changing climate. The diversity of the gibberellin signaling pathway genes involved in plant height control- ), ) and )-was studied in the diploid wild goatgrass Coss., one of the ancestral species of the bread wheat ( L.) and the donor of its D subgenome, using high-throughput sequencing. The examination of 24 accessions of different geographical origins revealed a large number of new alleles (haplotypes) not found in bread wheat varieties. Some of the detected polymorphisms lead to changes in the amino acid sequence of proteins. Four isoforms (amino acid sequence variants) were found for the RHT-D1 protein, and two isoforms-for the GID1 and GID2 proteins, each. An analysis of the co-occurrence frequencies of various isoforms of the three proteins showed that their combinations were not random in , which may indicate the functional significance of their differences. New alleles of the , , and genes are promising for introgression into bread wheat and studying their effect on plant height and adaptability.