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Obtaining Eggs. | LitMetric

Obtaining Eggs.

Cold Spring Harb Protoc

National Xenopus Resource, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA

Published: March 2021

AI Article Synopsis

  • Nearly a century ago, researchers like Lancelot Hogben discovered that injecting mammalian gonadotropins into the dorsal lymph sac of female animals can induce ovulation, allowing for year-round egg production.
  • * These hormonally induced females are able to produce thousands of eggs in a single spawning, which can be fertilized for embryos or used to create egg extracts.
  • * The protocol for this egg induction and collection is simple and varies slightly between labs, aiming to provide a clear method that researchers without previous experience can easily follow.

Article Abstract

Nearly a century ago, studies by Lancelot Hogben and others demonstrated that ovulation in female can be induced via injection of mammalian gonadotropins into the dorsal lymph sac, allowing for egg production throughout the year independent of the normal reproductive cycles. Hormonally induced females are capable of producing thousands of eggs in a single spawning, which can then be fertilized to generate embryos or used as a substrate for generation of egg extracts. The protocol for induction of ovulation and subsequent egg collection is straightforward and robust, yet some of its details may vary among laboratories based on prior training, availability of necessary reagents, or the experimental objectives. As the goal of this protocol is not to describe every single variation possible for acquiring eggs but to provide a simple and clear description that can be easily applied by researchers with no prior working experience with , we focus on describing the method we use at the National Resource-that is, inducing ovulation in via dorsal lymph sac injection of gonadotropic hormones and the stimulation of egg laying through application of gentle pressure to the females.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925371PMC
http://dx.doi.org/10.1101/pdb.prot106203DOI Listing

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