AI Article Synopsis
- African clawed frog embryos are valuable for studying various biological processes and diseases across multiple fields like neurobiology, physiology, and developmental biology.
- In vitro fertilization (IVF) is a method that allows the simultaneous fertilization of many eggs but typically results in the death of the male frog, which can be problematic for rare species.
- An alternative is natural mating, which gathers embryos without harming the male but might affect the timing and synchronization of embryo development.
Article Abstract
The embryos of the African clawed frog, , are a powerful substrate for the study of complex fundamental biological and disease mechanisms in neurobiology, physiology, molecular biology, cell biology, and developmental biology. A simple and straightforward technique for generating a large number of developmentally synchronized embryos is in vitro fertilization (IVF). IVF permits simultaneous fertilization of thousands of eggs but requires the death of the parental male, which may not be feasible if the male comes from a stock of precious animals. An alternative to euthanizing a precious male is to use a natural mating, which allows for the collection of many embryos with minimal preparation but with the potential loss of the experimental advantage of developmental synchronization. Here we present both strategies for obtaining embryos.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925375 | PMC |
| http://dx.doi.org/10.1101/pdb.prot106211 | DOI Listing |
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