AI Article Synopsis
- Cancer stem cells (CSCs) significantly contribute to colorectal cancer (CRC) development and treatment resistance, making them important targets for research.
- This study focuses on the expression of two markers, DCLK1 and Lgr5, in CRC patients to evaluate their potential as prognostic indicators for disease outcomes.
- Results show that high levels of DCLK1 and Lgr5 are linked to poorer survival rates and may indicate DCLK1 as a key factor for predicting CRC recurrence and overall patient survival.
Article Abstract
Background: Cancer stem cells (CSCs) are a subpopulation of cancer cells with the potential of self-renewal and differentiation. CSCs play critical roles in tumorigenesis, recurrence, metastasis, radiation tolerance and chemoresistance.
Aim: To assess the expression patterns and clinical potential of doublecortin-like kinase 1 (DCLK1) and leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5), as prognostic CSC markers of colorectal cancer (CRC).
Methods: The expression of DCLK1 and Lgr5 in CRC tissue sections from 92 patients was determined by immunohistochemistry. Each case was evaluated using a combined scoring method based on signal intensity staining (scored 0-3) and the proportion of positively stained cancer cells (scored 0-3). The final staining score was calculated as the intensity score multiplied by the proportion score. Low expression of DCLK1 and Lgr5 was defined as a score of 0-3; high expression of DCLK1 and Lgr5 was defined as a score of ≥ 4. Specimens were categorized as either high or low expression, and the correlation between the expression of DCLK1 or Lgr5 and clinicopathological factors was investigated.
Results: DCLK1 and Lgr5 expression levels were significantly positively correlated. CRC patients with high DCLK1, Lgr5 and DCLK1/Lgr5 expressions had poorer progression-free survival and overall survival. Moreover, high expression of DCLK1 was an independent prognostic factor for recurrence and overall survival in patients with CRC by multivariate analysis ( = 0.026 and = 0.049, respectively).
Conclusion: DCLK1 may be a potential CSC marker for the recurrence and survival of CRC patients.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7684452 | PMC |
| http://dx.doi.org/10.3748/wjg.v26.i43.6853 | DOI Listing |
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