AI Article Synopsis
- Zebrafish embryos offer a valuable model for studying complex biological processes, but traditional imaging techniques often disrupt their integrity.
- This study introduces confocal Raman spectroscopic imaging, which allows researchers to visualize and analyze biomolecular information from unlabeled zebrafish embryos without damaging them.
- The method has versatile applications, including 3D visualization of biomolecular distribution, resolving subcellular features, profiling Mycobacterium marinum strains, and monitoring wound responses in live embryos.
Article Abstract
Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from unlabeled zebrafish embryos. We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivo temporal monitoring of the wound response in living zebrafish embryos. Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis of fully intact and living zebrafish embryos.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710741 | PMC |
| http://dx.doi.org/10.1038/s41467-020-19827-1 | DOI Listing |
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