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Rapid and Robust Monitoring of Phytophthora Infectivity Through Detached Leaf Assays with Automated Image Analysis.
Methods Mol Biol
December 2024
School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
The detached leaf assay is a valuable method for studying plant-pathogen interactions, enabling the assessment of pathogenicity, plant resistance, and treatment effects. In this protocol, we outline how to set up a Phytophthora detached leaf assay and use non-expert machine learning tools to increase the reliability and throughput of the image analysis. Utilizing ilastik for pixel classification and Python scripts for segmentation, manual correction, and temporal linking, the pipeline provides objective and quantitative data over time.
View Article and Find Full Text PDFPhysician vs. AI-generated messages in urology: evaluation of accuracy, completeness, and preference by patients and physicians.
World J Urol
December 2024
Department of Urology, Baldwin Park Medical Center, Kaiser Permanente, 1011 Baldwin Park Blvd., Baldwin Park, CA, 91706, USA.
Purpose: To evaluate the accuracy, comprehensiveness, empathetic tone, and patient preference for AI and urologist responses to patient messages concerning common BPH questions across phases of care.
Methods: Cross-sectional study evaluating responses to 20 BPH-related questions generated by 2 AI chatbots and 4 urologists in a simulated clinical messaging environment without direct patient interaction. Accuracy, completeness, and empathetic tone of responses assessed by experts using Likert scales, and preferences and perceptions of authorship (chatbot vs.
Psychometric Properties of the Lactation Assessment and Comprehensive Intervention Tool (LAT).
Nurs Rep
December 2024
Healthy Children Project, Inc., Harwich, MA 02645, USA.
Background: Despite the short- and long-term acknowledged benefits of breastfeeding for mothers and their infants, worldwide rates trail behind international goals. Prior research confirms that breastfeeding is a nurse sensitive indicator and that problems with latching the baby and painful breastfeeding rank high among the reasons given for not continuing to breastfeed. The Lactation Assessment Tool (LAT) was previously evaluated in a study conducted in Latvia by nurse midwives.
View Article and Find Full Text PDFManagement of Graves' upper eyelid retraction (GUER): A review.
Indian J Ophthalmol
December 2024
Hariram Motumal Nasta and Renu Hariram Nasta Ophthalmic Plastic Surgery Service, L.V. Prasad Eye Institute, Hyderabad, Telangana, India.
Graves' disease, a common autoimmune disorder, characteristically presents with upper eyelid retraction, causing significant functional and cosmetic concerns for affected individuals. The management of Graves' upper eyelid retraction has evolved significantly over recent years, with various surgical and non-surgical interventions. An ideal procedure is predictable and easily repeatable.
View Article and Find Full Text PDFEffects of Acute Stress on Metabolic Interactions Related to the Tricarboxylic Acid (TCA) Cycle in the Left Hippocampus of Mice.
Metabolites
December 2024
Department of Radiation Convergence Engineering, College of Software and Digital Healthcare Convergence, Yonsei University, 1, Yeonsedae-gil, Heungeop-myeon, Wonju 26493, Republic of Korea.
Background/objectives: The acute stress response affects brain metabolites closely linked to the tricarboxylic acid (TCA) cycle. This response involves time-dependent changes in hormones and neurotransmitters, which contribute to resilience and the ability to adapt to acute stress while maintaining homeostasis. This physiological mechanism of metabolic dynamics, combined with time-series analysis, has prompted the development of new methods to observe the relationship between TCA cycle-related brain metabolites.
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