sp. nov., a marine bacterium isolated from tidal flat sediment and emended description of the genus Hameed . 2015.

A Gram-stain-negative, facultatively anaerobic, rod-shaped (1.8-4.4×0.5-0.7 µm) and motile marine bacterium, designated as MEBiC13590, was isolated from tidal flat sediment sampled at Incheon City, on the west coast of the Republic of Korea. The 16S rRNA gene sequence analysis revealed that strain MEBiC13590 showed high similarity to CC-AMH-0 (98.2 %), followed by StC1 (97.5 %); however, it clustered with . The phylogenomic tree inferred by the up-to-date bacterial core gene set suggested that strain MEBiC13590 shared a phyletic line with . Average nucleotide identity and digital DNA-DNA hybridization values (75.0 and 19.3 %, respectively) between strain MEBiC13590 and CC-AMH-0 were below the respective species delineation cutoffs. Growth was observed at 22-50 °C (optimum, 45 °C), at pH 5-9 (optimum, pH 7) and with 1-6 % (optimum, 3 %) NaCl. The predominant cellular fatty acids were C (7.6 %), C (12.2 %), 11-methyl C ω7 (5.7 %), C cyclo ω6 and summed feature 8 (comprising C ω7 and/or C ω6; 38 %). The DNA G+C content was 63.5 mol%. The major respiratory quinone was Q-10. Several phenotypic characteristics such as growth temperature, oxygen requirement, enzyme activities of urease, gelatinase, lipase (C), α-chymotrypsin, acid phosphatase, β-galactosidase, β-glucosidase etc. differentiate strain MEBiC13590 from CC-AMH-0. Based on this polyphasic taxonomic data, strain MEBiC13590 should be classified as representing a novel species in the genus for which the name sp. nov. is proposed . The type strain is MEBiC13590 (=KCCM 43313=JCM 33661).