AI Article Synopsis
- Polysaccharides are the most common natural biopolymers, used in various sectors like food, medicine, and cosmetics.
- The physicochemical properties, such as particle size and molecular weight, of these polysaccharides impact their applications.
- Asymmetrical flow field-flow fractionation (AF4) has emerged as a key method for separating and characterizing polysaccharides, offering advantages like less shear degradation, and this review discusses its principles and applications over the last decade.
Article Abstract
Polysaccharides are the most abundant natural biopolymers on the earth and are widely used in food, medicine, materials, cosmetics, and other fields. The physicochemical properties of polysaccharides such as particle size and molecular weight often affect their practical applications. In recent years, asymmetrical flow field-flow fractionation (AF4) has been widely used in the separation and characterization of polysaccharides because it has no stationary phases or packing materials, which reduces the risk of shear degradation of polysaccharides. In this review, the principle of AF4 was introduced briefly. The operation conditions of AF4 for the analysis of polysaccharides were discussed. The applications of AF4 for the separation and characterization of polysaccharides from different sources (plants, animals, and microorganisms) over the last decade were critically reviewed.
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| http://dx.doi.org/10.1016/j.chroma.2020.461726 | DOI Listing |
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