Gammaproteobacterium Thalassolituus oleivorans plays an important role in oil degradation in sea water through emulsifying crude oil and alkanes at low temperatures in polar sea environment. Here we report the complete genome sequence of K-188 strain (VKPM B-9394) isolated in the Barents Sea and compare it with other known Thalassolituus oleivorans strains. The Thalassolituus strains are differed in orthologs number of the genes of alkane degradation, transport proteins, genes of sugar utilization, endonucleases, signaling proteins, transcriptional regulators and presence of CRISPR/Cas locus. Also only the genome of K-188 contains the 3-hydroxyalkanoate synthetase.
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http://dx.doi.org/10.1016/j.margen.2017.08.005 | DOI Listing |
Mol Biol Rep
October 2024
University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, South China Agricultural University, Guangzhou, 510642, China.
Background: The Pearl River and its estuary are highly exposed to anthropogenic disturbance. Because bacterial communities play an indispensable role in aquatic ecosystems, there has been an increased research focus on the statuses of these communities under human-induced perturbations.
Methods And Results: This study investigated the composition, diversity, and structure of bacterial communities across 29 sites from the Guangzhou section of the Pearl River (GZ) to the Pearl River Estuary (PRE) using 16S rRNA gene amplicons.
Int J Syst Evol Microbiol
May 2022
Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, School of Life Sciences, Fudan University, Shanghai, PR China.
Two strains, TMPB967 and TTPB476, were isolated from two different locations in the Mariana Trench. Cells of strains TMPB967 and TTPB476 were Gram-negative, curved rod-shaped (0.35-0.
View Article and Find Full Text PDFFront Microbiol
December 2018
School of Biological Sciences, University of Essex, Colchester, United Kingdom.
The marine obligate hydrocarbonoclastic bacterium MIL-1 metabolizes a broad range of aliphatic hydrocarbons almost exclusively as carbon and energy sources. We used LC-MS/MS shotgun proteomics to identify proteins involved in aerobic alkane degradation during growth on medium- (-C) or long-chain (-C) alkanes. During growth on -C, expresses an alkane monooxygenase system involved in terminal oxidation including two alkane 1-monooxygenases, a ferredoxin, a ferredoxin reductase and an aldehyde dehydrogenase.
View Article and Find Full Text PDFMar Genomics
February 2018
Vavilov Institute of General Genetics, Gubkina str., h. 3, Moscow 119991, Russian Federation; State Institute for Genetics and Selection of Industrial Microorganisms, 1-st Dorozhniy pr., h. 1, Moscow 117545, Russian Federation; Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141700, Russian Federation. Electronic address:
Gammaproteobacterium Thalassolituus oleivorans plays an important role in oil degradation in sea water through emulsifying crude oil and alkanes at low temperatures in polar sea environment. Here we report the complete genome sequence of K-188 strain (VKPM B-9394) isolated in the Barents Sea and compare it with other known Thalassolituus oleivorans strains. The Thalassolituus strains are differed in orthologs number of the genes of alkane degradation, transport proteins, genes of sugar utilization, endonucleases, signaling proteins, transcriptional regulators and presence of CRISPR/Cas locus.
View Article and Find Full Text PDFJ Environ Sci (China)
May 2016
Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Messina, Messina, Italy.
In order to better understand the effects of biostimulation and bioaugmentation processes on a marine microbial community, three different mesocosm experiments were planned. Natural seawater (10.000L) was artificially polluted with crude oil (1L) and (1) inorganic nutrients (Biostimulating Mesocosm, BM), (2) inorganic nutrients and an inoculum of Alcanivorax borkumensis SK2 (Single Bioaugmentation Mesocosm, SBM), (3) inorganic nutrients and inoculums of A.
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