Effects of seawater acclimation on two Na/K-ATPase α-subunit isoforms in the gills of the marble goby, Oxyeleotris marmorata.

Comp Biochem Physiol A Mol Integr Physiol

Natural Sciences and Science Education, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Republic of Singapore. Electronic address:

Published: March 2021

AI Article Synopsis

  • The marble goby can adapt from freshwater to seawater, displaying unique physiological traits including the ability to survive without water for extended periods.
  • Two specific isoforms of Na/K-ATPase (nka), nkaα1 and nkaα3, have been identified in its gills, with nkaα1 being more abundant than nkaα3, particularly in freshwater.
  • Acclimation to seawater significantly enhances the expression levels of these isoforms at both mRNA and protein levels, indicating their crucial role in the fish's adaptation process.

Article Abstract

The marble goby, Oxyeleotris marmorata, is a freshwater teleost, but can acclimate progressively to survive in seawater (salinity 30). As an obligatory air-breather, it can also survive long periods of emersion. Two isoforms of Na/K-ATPase (nka) α-subunit, nkaα1 and nkaα3, but not nkaα2, had been cloned from the gills of O. marmorata. The cDNA sequence of nkaα1 consisted of 3069 nucleotides, coding for 1023 amino acids (112.5 kDa), whereas nkaα3 consisted of 2976 nucleotides, coding for 992 amino acids (109.5 kDa). As only one form of branchial Nkaα1 was identified using molecular cloning in this study, O. marmorata lacks specific freshwater- and seawater-type Nkaα isoforms as demonstrated by some other euryhaline fish species. The nkaα1 transcript level was about 2.5-fold higher than that of nkaα3 in the gills of freshwater O. marmorata. During exposure to seawater, the branchial transcript level of nkaα1 increased significantly on day 1 (~3.3-fold) and day 6 (~2.6-fold). By contrast, the branchial transcript level of nkaα3 increased significantly on day 1 (~2.6-fold), but not on day 6, of seawater exposure. Six days of exposure to seawater also led to significant increases in protein abundances of Nkaα1 (~6.9-fold) and Nkaα3 (~2.8-fold) in the gills of O. marmorata. Hence, the mRNA and protein expressions of both nkaα1/Nkaα1 and nkaα3/Nkaα3 were up-regulated in O. marmorata during seawater acclimation. This could explain why V increases but K for Na and K remain unchanged in Nka extracted from the gills of O. marmorata acclimated to seawater as reported previously.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbpa.2020.110853DOI Listing

Publication Analysis

Top Keywords

gills marmorata
12
transcript level
12
seawater acclimation
8
marble goby
8
goby oxyeleotris
8
marmorata
8
oxyeleotris marmorata
8
nucleotides coding
8
amino acids
8
exposure seawater
8

Similar Publications

Article Synopsis
  • The study investigates how transposable elements (TEs) in fish gill transcriptomes respond to varying salinity levels in three different species: giant marbled eel, chum salmon, and marine medaka.
  • Findings show that juvenile eels exhibit significant TE activity when transitioning between salty and freshwater environments.
  • The research also identifies a unique krüppel-associated box (KRAB)-like domain in fish, suggesting a potential interaction with TRIM33 that may enable the NuRD complex's function in these species.
View Article and Find Full Text PDF

Recently, the culture of American eels (Anguilla rostrate) in China has been impacted by emergence of a disease with signs of haemorrhagic gill necrosis. The gills of diseased eels are covered with petecchia and they bleed when the operculum is pressed. In this study, a novel American eel adomavirus (AEAdoV) was isolated from the diseased eels using the eel ovary cell line (EO).

View Article and Find Full Text PDF

Environmental salinity influences the branchial expression of TCR pathway related genes based on transcriptome of a catadromous fish.

Comp Biochem Physiol Part D Genomics Proteomics

June 2021

College of Marine Science and Engineering, College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang 222005, China. Electronic address:

Environmental salinity not only affects the physiological processes such as osmoregulation and hormonal control, but also changes the immune system in fishes. Studies are limited in fish on the roles of the T cell receptor (TCR)-related genes in relation to changes in environmental salinity. A large group of salinity-challenged transcripts was obtained in gills of marbled eel (Anguilla marmorata).

View Article and Find Full Text PDF

The global invasive anguillid gill parasite Pseudodactylogyrus anguillae (Yin and Sproston, 1948) has only recently been documented from eels in South Africa. As there is no known eel trade in South Africa, the source of introduction of this parasite has been debated, and its status as an alien parasite was rendered uncertain. We report on the first infection of Pseudodactylogyrus anguillae from the giant mottled eel Anguilla marmorata from the Phongolo River (South Africa) using classic morphological and molecular methodologies and clarify the introduction status category of this parasite as alien and invasive.

View Article and Find Full Text PDF

Effects of seawater acclimation on two Na/K-ATPase α-subunit isoforms in the gills of the marble goby, Oxyeleotris marmorata.

Comp Biochem Physiol A Mol Integr Physiol

March 2021

Natural Sciences and Science Education, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Republic of Singapore. Electronic address:

Article Synopsis
  • The marble goby can adapt from freshwater to seawater, displaying unique physiological traits including the ability to survive without water for extended periods.
  • Two specific isoforms of Na/K-ATPase (nka), nkaα1 and nkaα3, have been identified in its gills, with nkaα1 being more abundant than nkaα3, particularly in freshwater.
  • Acclimation to seawater significantly enhances the expression levels of these isoforms at both mRNA and protein levels, indicating their crucial role in the fish's adaptation process.
View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!