The ability of bacterial pathogens to form biofilms is an important virulence mechanism in relation to their pathogenesis and transmission. Biofilms play a crucial role in survival in unfavorable environmental conditions, acting as reservoirs of microbial contamination and antibiotic resistance. For intestinal pathogen , biofilms are considered to be a contributing factor in transmission through the food chain and currently, there are no known methods for intervention. Here, we present an unconventional approach to reducing biofilm formation by by the application of D-amino acids (DAs), and L-amino acids (LAs). We found that DAs and not LAs, except L-alanine, reduced biofilm formation by up to 70%. The treatment of cells with DAs changed the biofilm architecture and reduced the appearance of amyloid-like fibrils. In addition, a mixture of DAs enhanced antimicrobial efficacy of D-Cycloserine (DCS) up to 32% as compared with DCS treatment alone. Unexpectedly, D-alanine was able to reverse the inhibitory effect of other DAs as well as that of DCS. Furthermore, L-alanine and D-tryptophan decreased transcript levels of peptidoglycan biosynthesis enzymes alanine racemase () and D-alanine-D-alanine ligase () while D-serine was only able to decrease the transcript levels of . Our findings suggest that a combination of DAs could reduce biofilm formation, viability and persistence of through dysregulation of and
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http://dx.doi.org/10.3390/antibiotics9110836 | DOI Listing |
Microbiol Spectr
January 2025
Department of Veterinary Science, Martin-Gatton College of Agriculture, Food, and Environment, University of Kentucky, Lexington, Kentucky, USA.
Unlabelled: is a foodborne pathogen that poses a significant threat to global public health. It affects several animal species, including horses. infections in horses can be either asymptomatic or cause severe clinical illness.
View Article and Find Full Text PDFMed Sci (Basel)
January 2025
Medical and Pharmaceutical Sciences Group, Faculty of Health Sciences, University of Sucre, Sincelejo 700001, Sucre, Colombia.
Background/objectives: is a clinically significant opportunistic pathogen, renowned for its ability to acquire and develop diverse mechanisms of antibiotic resistance. This study examines the resistance, virulence, and regulatory mechanisms in extensively drug-resistant clinical strains of .
Methods: Antibiotic susceptibility was assessed using the Minimum Inhibitory Concentration (MIC) method, and whole-genome sequencing (WGS) was performed on the Illumina NovaSeq platform.
Zool Res
January 2025
Fisheries College, Jimei University, Xiamen, Fujian 361021, China. E-mail:
Small RNAs (sRNAs) are a class of molecules capable of perceiving environmental changes and exerting post-transcriptional regulation over target gene expression, thereby influencing bacterial virulence and host immune responses. is a pathogenic bacterium that poses a significant threat to aquatic animal health. However, the regulatory mechanisms of sRNAs in .
View Article and Find Full Text PDFBiofouling
January 2025
Department of Food Technology, Universidade Federal de Viçosa, Viçosa, Brazil.
The dairy industry faces challenges in controlling spoilage microorganisms, particularly , known to form resilient biofilms. Conventional disinfection methods have limitations, prompting the exploration of eco-friendly alternatives like ozone. This study focused on biofilms on polystyrene and polyethylene surfaces, evaluating ozone efficacy when incorporated into different water sources and applied under static and dynamic conditions.
View Article and Find Full Text PDFBiofilm
June 2025
Instituto de Productos Lácteos de Asturias (IPLA-CSIC)C/ Francisco Pintado Fe 26, 33011, Oviedo, Asturias, Spain.
Environmental cues sometimes have a direct impact on phage particle stability, as well as bacterial physiology and metabolism, having a profound effect on phage infection outcome. Here, we explore the impact of temperature on the interplay between phage (phiIPLA-RODI) and its host, . Our results show that phiIPLA-RODI is a more effective predator at room (25 °C) compared to body temperature (37 °C) against planktonic cultures of several strains with varying degrees of phage susceptibility.
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