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Neurofilament light chain (Nf-L) in cerebrospinal fluid and serum as a potential biomarker in the differential diagnosis of neurological diseases in cattle.
Vet Res
January 2025
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2-5, 10095, Grugliasco, TO, Italy.
Article Synopsis
- Neurofilament light chain (Nf-L) is identified as a potential biomarker for diagnosing neurological disorders in cattle, paralleling its established role in human neurology.
- The study found that Nf-L levels varied significantly between healthy and sick cattle, with consistent median levels detected in serum and cerebrospinal fluid (CSF) across different age groups.
- There is a promising association between Nf-L levels in serum and CSF, particularly in cattle with neurological disorders, indicating that Nf-L could serve as a valuable diagnostic tool in veterinary practices.
Shared decision-making in type 2 diabetes: a systematic review of patients' preferences and healthcare providers' perspectives.
BMC Health Serv Res
January 2025
Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Background: Shared decision-making (SDM) is crucial for type 2 diabetes mellitus (T2DM) management due to the complexity of treatment options. This systematic review sought to understand T2DM patients' preferences and diabetes care providers' perspectives regarding SDM, and the barriers and facilitators to SDM.
Methods: Five databases were searched from 2000 to 2023 (Medline/PubMed, Web of Science, Scopus, PsycINFO, and Embase).
Modulates Dopamine to Increase Sugar Responsiveness in Honeybees ().
Int J Mol Sci
December 2024
College of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
The appetite of honeybees for food is crucial to their survival and reproduction, as they sustain their entire colony by collecting pollen and nectar for nutrients. Dopamine, an important neurotransmitter, regulates appetite and satiety. However, how dopamine regulates honeybee foraging behavior remains unexplored.
View Article and Find Full Text PDFWanted: Dead or Alive Cells with Propidium Iodide Staining in Liver Tissue.
Int J Mol Sci
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Goethe University, Frankfurt University Hospital, Medical Clinic 1, 60596 Frankfurt, Germany.
This study demonstrates the effectiveness of propidium iodide as a reliable marker for detecting dead or dying cells in frozen liver tissue sections. By comparing propidium iodide staining with the widely used Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, both methods showed consistent results in disease models such as alcohol-induced fibrosis and Western diet-induced fatty liver. Additionally, propidium iodide was successfully co-stained with other fluorescent markers, like phalloidin (for actin filaments) and antibodies targeting collagen, enabling detailed spatial analysis of dying cells within tissue.
View Article and Find Full Text PDFSelf-esteem, defense mechanisms, sexual satisfaction and stress coping mechanisms in individuals treated for vaginismus: A controlled study.
Afr J Reprod Health
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Faculty of Medicine, Kocaeli University, Turkey.
The aim of study was to analyze the extent to which treatment for vaginismus affect self-esteem, defense mechanisms, sexual satisfaction and coping with stress. Was conducted as aquasi-experimental, cross-sectional study. The population of the study consisted of womenwho were followed up with the diagnosis of vaginismus in obstetrics clinic.
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