Mouse models of allergic conjunctivitis mimic various aspects of human allergic conjunctivitis. They are useful as acute models of allergic conjunctivitis to study immunological aspects of this condition. In this chapter, we will describe ragweed-pollen-induced experimental allergic conjunctivitis (mostly driven by adaptive immunity), and papain-soaked contact lens-induced experimental allergic conjunctivitis (mostly driven by innate immunity). Giemsa staining of histological sections is used for quantification of the number of infiltrating eosinophils, which is useful to evaluate the severity of the allergic inflammation. Immunohistochemical staining and quantitative PCR are used to clarify spatiotemporal expression of proinflammatory molecules in the conjunctival tissue. Flow cytometric analysis of conjunctival tissue is used for the detection of innate lymphoid cell type 2 (ILC2) in the ocular surface tissues.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-1001-5_10 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Climate change and ocular surface diseases].
Ophthalmologie
January 2025
Klinik für Augenheilkunde, Universitätsklinikum Düsseldorf, Moorenstr. 5, 40225, Düsseldorf, Deutschland.
Background: The ocular surface is directly exposed to environmental influences. Noxae that have already been identified for the ocular surface are heat, air dryness, pollutant gases, fine dust particles and ultraviolet radiation.
Methods: The current literature was used to investigate the relationship between frequent ocular surface diseases and various environmental factors and to analyze their development over the years.
The Role of In Vivo Confocal Microscopy in Ocular Allergies.
Turk J Ophthalmol
December 2024
Keio University Faculty of Medicine, Department of Ophthalmology, Tokyo, Japan.
In vivo confocal microscopy (IVCM) is a non-invasive imaging technique used to visualize the layers of the cornea and conjunctiva in real time. In patients with atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC), this technology can be useful in diagnosing and monitoring the disease, as well as evaluating the efficacy of treatments. IVCM can reveal subclinical abnormalities in the corneal and conjunctival epithelium such as inflammatory cell infiltrates and tissue damage, which can provide insight into the pathogenesis of AKC.
View Article and Find Full Text PDFPurpose: To study the epidemiology and clinical presentation of allergic eye diseases (AEDs) and Vernal Keratoconjunctivitis (VKC) METHODS: A cross-sectional- cum-cohort study was conducted in rural and urban areas in different geographical locations (plains, hilly, high-altitude and coastal) in India. Children (5-15 years) were included, information on exposure to environmental factors gathered, participants screened for AED and VKC on torch light, followed by a comprehensive eye examination. Cases were compared with controls.
View Article and Find Full Text PDFThe Spectrum of Ocular Diseases in the Onchocerciasis-Endemic Focus of Raga in South Sudan.
Res Rep Trop Med
December 2024
Global Health Institute, University of Antwerp, Antwerp, Belgium.
Introduction: Raga County is an onchocerciasis-endemic area in the Western Bahr El Ghazal state of South Sudan, known to have a high prevalence of blindness. The objective of this study was to determine the causes of eye disease and blindness in Raga County as well as to assess the relationship of eye diseases with other prevalent conditions like onchocerciasis and epilepsy.
Methods: We reviewed unpublished pre-community directed treatment with ivermectin (CDTI) data about eye disease and onchocerciasis in Western Bahr El Ghazal including Raga.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!