AI Article Synopsis
- Counting chromosomes is an essential but often overlooked technique in botany; it can be applied to many types of plants and research questions with just basic equipment.
- The article outlines a detailed protocol for preparing angiosperm pollen mother cells, including guidance on choosing, staining, and squashing, along with figures and demonstration videos.
- The goal is to make this valuable technique more accessible and encourage its use among researchers, regardless of their available resources.
Article Abstract
Premise: Counting chromosomes is a fundamental botanical technique, yet it is often intimidating and increasingly sidestepped. Once mastered, the basic protocol can be applied to a broad range of taxa and research questions. It also reveals an aspect of the plant genome that is accessible with only the most basic of resources-access to a microscope with 1000× magnification is the most limiting factor.
Methods And Results: Here we provide a detailed protocol for choosing, staining, and squashing angiosperm pollen mother cells. The protocol is supplemented by figures and two demonstration videos.
Conclusions: The protocol we provide will hopefully demystify and reinvigorate a powerful and once commonplace botanical technique that is available to researchers regardless of their location and resources.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667484 | PMC |
| http://dx.doi.org/10.1002/aps3.11342 | DOI Listing |
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