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A fast and responsive turn-on fluorescent probe based on a quinone conjugated alkoxy derivative for biothiols and a cellular imaging study.
Turk J Chem
November 2024
Division of Organic Chemistry, Department of Chemistry, Faculty of Engineering, İstanbul University-Cerrahpaşa, İstanbul, Turkiye.
The detection of intracellular biothiols (cysteine, N-acetyl cysteine, and glutathione) with high selectivity and sensitivity is important to reveal biological functions. In this study, a 2-(2-methoxy-4-methylphenoxy)-3-chloro-5,8-dihydroxynaphthalene-1,4-dione (DDN-O) compound was newly synthesized and used as a fluorogenic probe (detector molecule) in the fluorometric method for the rapid, highly selective, and sensitive determination of biothiols. The intensity values (λ = 260 nm, λ = 620 nm) of the product were measured by adding biothiols to the reaction medium at varying concentrations and the glutathione equivalent thiol content values of each biothiol were calculated.
View Article and Find Full Text PDFA handheld biofluorometric system for acetone detection in exhaled breath condensates.
Analyst
January 2025
Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
As a marker of human metabolism, acetone is important for lipid metabolism monitoring and early detection of diabetes. In this study, we developed a handheld biosensor for acetone based on fluorescence detection by utilizing the enzymatic reaction of secondary alcohol dehydrogenase (S-ADH) with β-nicotinamide adenine dinucleotide (NADH, = 340 nm, = 490 nm). In the reaction, NADH is oxidized when acetone is reduced to 2-propanol by S-ADH, and the acetone concentration can be measured by detecting the amount of NADH consumed in this reaction.
View Article and Find Full Text PDFArchitecture of a novel NIR and switch-on fluorescent chemosensor based on mercapto propanehydrazide-functionalized chromenylium-cyanine for the quantification of mercury(II) in environment and living cells.
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
Istanbul Technical University, Department of Chemistry, 34469 Maslak, Istanbul, Turkey. Electronic address:
A novel probe NIR8 decorated with colorimetric, fluorometric, turn-on, and NIR properties was designed and synthesized using the chromenylium-cyanine platform for sensitive and selective quantification of Hg in real samples. The interaction between the probe and Hg resulted in the opening of the closed spirolactam ring of the chromenylium-cyanine platform, leading to a notable color shift from yellow to green. Concurrently, ratiometric spectral changes at 380 and 725 nm for UV-Vis and signal enhancement at 755 nm for fluorescence were observed.
View Article and Find Full Text PDFExcited state intramolecular proton transfer (ESIPT) termination enables "one-to-two" sensing property of an indolocarbazole-based Schiff base.
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, PR China. Electronic address:
Detections towards multiple analytes by one sensor remain a significant challenges. Herein, a high-sensitivity chemo-sensor ICZ-o-XFJ is designed and synthesized for the simultaneous detection of F and Al. Suffering from the ESIPT process, this sensor is colorless and almost non-emissive in solutions.
View Article and Find Full Text PDFCurcumin-Derived (3-Aminopropyl)trimethoxysilane-Functionalized Carbon Quantum Dots: A Fluorometric and Colorimetric Nanoprobe for Picric Acid Detection, Antioxidant Activity, and Liposome Encapsulation.
ACS Appl Mater Interfaces
December 2024
Department of Chemistry, Indian Institute of Technology (BHU), Varanasi 221005, Uttar Pradesh, India.
Creating an analytical probe to track extremely mutagenic picric acid (PA) is essential for human health and the environment. Here, we developed a straightforward and quick fluorescence analytical method utilizing 3-aminopropyltrimethoxysilane (3-APTMS)-functionalized curcumin carbon quantum dots (CQDs) for the fast and selective detection of PA. Solvothermal carbonization and functionalization of curcumin with 3-APTMS were used to create multifunctional CQDs, which were then characterized using UV-vis spectroscopy, Fourier transform infrared (FTIR), X-ray diffraction (XRD), ζ-potential, transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS).
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