Prenatal ethanol exposure can result in loss of neural stem cells (NSCs) and decreased brain growth. Here, we assessed whether a noncoding RNA (ncRNA) related to the NSC self-renewal factor Oct4/Pou5f1, and transcribed from a processed pseudogene locus on mouse chromosome 9 (mOct4pg9), contributed to the loss of NSCs due to ethanol. Mouse fetal cortical-derived NSCs, cultured ex vivo to mimic the early neurogenic environment of the fetal telencephalon, expressed mOct4pg9 ncRNA at significantly higher levels than the parent Oct4/Pou5f1 mRNA. Ethanol exposure increased expression of mOct4pg9 ncRNA, but decreased expression of Oct4/Pou5f1. Gain- and loss-of-function analyses indicated that mOct4pg9 overexpression generally mimicked effects of ethanol exposure, resulting in increased proliferation and expression of transcripts associated with neural maturation. Moreover, mOct4pg9 associated with Ago2 and with miRNAs, including the anti-proliferative miR-328-3p, whose levels were reduced following mOct4pg9 overexpression. Finally, mOct4pg9 inhibited Oct4/Pou5f1-3'UTR-dependent protein translation. Consistent with these observations, data from single-cell transcriptome analysis showed that mOct4pg9-expressing progenitors lack Oct4/Pou5f1, but instead overexpress transcripts for increased mitosis, suggesting initiation of transit amplification. Collectively, these data suggest that the inhibitory effects of ethanol on brain development are explained, in part, by a novel ncRNA which promotes loss of NSC identity and maturation.
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http://dx.doi.org/10.1016/j.ntt.2020.106943 | DOI Listing |
J Econ Entomol
January 2025
Department of Entomology, University of Georgia, Griffin, GA, USA.
Xylosandrus crassiusculus Motschulsky and Xylosandrus germanus Blandford are serious ambrosia beetle pests in ornamental nurseries. Three ethanol baits, AgBio low release (LR), AgBio high release (HR), and Trécé are commercially available for use in bottle traps to determine flight activity of adult Xylosandrus spp. However, release patterns of ethanol from these baits under varying temperatures and captures of Xylosandrus spp.
View Article and Find Full Text PDFLangmuir
January 2025
Department of Chemical and Pharmaceutical Engineering, Faculty of Chemistry and Pharmacy, Sofia University, 1 James Bourchier Avenue, Sofia 1164, Bulgaria.
Spontaneous bubble growths in liquids are usually triggered by rapid changes in pressure or temperature that can lead to liquid gas supersaturation. Here, we report alternative scenarios of the spontaneous growths of bubbles inside a high-saturation-vapor-pressure and high-air-solubility perfluorocarbon liquid (PP1) that were observed under ambient quiescent conditions. First, we investigate spontaneous bubble growth inside the single PP1 phase, which was left to evaporate freely.
View Article and Find Full Text PDFNanomaterials (Basel)
January 2025
Department of Conservative Dentistry, University Hospital, Ludwig-Maximilians-University, Goethestr. 70, D-80336 Munich, Germany.
Objective: It is hypothesized that the way nano- and micro-hybrid polymer-based composites are structured and cured impacts the way they respond to aging.
Material And Methods: A polymer-ceramic interpenetrating network composite (Vita Enamic/VE), an industrially polymerized (Brillinat CriosST/BC), and an in situ light-cured composite with discrete inorganic fillers (Admira Fusion5/AF5) were selected. Specimens (308) were either cut from CAD/CAM blocks (VE/BC) or condensed and cured in white polyoxymethylene molds (AF5) and subjected to four different aging conditions ( = 22): (a) 24 h storage in distilled water at 37 °C; (b) 24 h storage in distilled water at 37 °C followed by thermal cycling for 10,000 cycles 5/55 °C (TC); (c) TC followed by storage in a 75% ethanol-water solution; and (d) TC followed by a 3-week demineralization/remineralization cycling.
ChemistryOpen
January 2025
Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria, Brazil.
This study investigates the antioxidant, antimicrobial, and anticancer properties of Pancratium maritimum L. in Sp. Pl.
View Article and Find Full Text PDFBreast Cancer Res
January 2025
Department of Epidemiology (EM, JEB) and Nutrition (KJM), Harvard T.H. Chan School of Public Health, 677 Huntington Ave, Kresge 505-B, Boston, MA, 02115, USA.
Background: Alcohol intake is associated with a higher risk of estrogen receptor-positive (ER+) breast cancer (BC), presumably through its confirmed ability to increase sex hormone levels. Whether consuming alcohol within the recommended limit of one serving per day increases sex hormone levels among postmenopausal women taking aromatase inhibitors (AI) to inhibit estrogen production remains unknown. Therefore, we compared sex hormone levels following white wine to levels following white grape juice among ER + BC survivors taking AIs.
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