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Diversity of Escherichia coli strains isolated from day-old broiler chicks, their environment and colibacillosis lesions in 80 flocks in France. | LitMetric

AI Article Synopsis

  • - Avian colibacillosis is a widespread bacterial infection in broilers, prompting a study on 80 flocks to understand the types and origins of E. coli strains responsible for these infections.
  • - Samples were taken from various sources, including the farm environment and day-old chicks, leading to the analysis of 967 E. coli isolates using high-throughput qPCR, which revealed a significant genetic variety.
  • - The findings indicated that while some isolates shared genetic profiles between diseased and healthy birds, many factors contributed to the diversity of E. coli strains, showing that not all contaminated chicks resulted in disease outbreaks.

Article Abstract

Avian colibacillosis is the most common bacterial disease affecting broilers. To better evaluate the diversity and the origin of the causative Escherichia coli strains infecting birds, we conducted a study on 80 broiler flocks. Just before the arrival of chicks on the farm, samples were collected in the farm environment (walls, feeders, air inlets, etc.) and, upon delivery, day-old chicks (DOCs) and the transport boxes were also sampled. Isolates were obtained from these samples, and from organs of chickens exhibiting typical colibacillosis symptoms. The isolates were characterized using high-throughput qPCR to detect a range of genetic markers (phylogroups, main serogroups virulence markers, etc.). A total of 967 isolates were studied, including 203 from 28 colibacillosis episodes, 484 from DOCs, 162 from transport boxes and 118 from the farm environment. These isolates yielded 416 different genetic profiles, of which 267 were detected in single isolates, and the others were observed in up to 44 isolates from nine farms. The distributions of isolates across phylogroups and the main serogroups varied with the origin of isolation. The isolates obtained from colibacillosis cases either shared a single genetic profile or were different. In a few cases, we observed the same profile for isolates obtained from DOCs and colibacillosis lesions in the same flock or different flocks. However, some flocks receiving DOCs contaminated with isolates bearing the genetic profile of colibacillosis cases identified in other flocks remained healthy. This study highlights the huge diversity among avian E. coli isolated from diseased and non diseased birds.

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Source
http://dx.doi.org/10.1016/j.vetmic.2020.108923DOI Listing

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