The study of metagenomics from high throughput sequencing data processed through Waikato Environment for Knowledge Analysis (WEKA) is gaining momentum in recent years. Therefore, we report an analysis of metagenome data generated using T-RFLP followed by using the SMO (Sequential minimal optimization) algorithm in WEKA to identify the total amount of cultured and uncultured microorganism present in the sample collected from multiple sources.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7649025 | PMC |
http://dx.doi.org/10.6026/97320630016620 | DOI Listing |
J Virol Methods
September 2024
CADRAD, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
Elephant endotheliotropic herpesviruses (EEHV) belong to the family Herpesviridae and cause a highly fatal hemorrhagic infection in elephants. EEHV poses a global threat to the already endangered elephant population. Since EEHV is a non-cultivable virus, there is a scarcity of specific diagnostics, therapeutics, and vaccines.
View Article and Find Full Text PDFserogroup B (NmB) strains have diverse antigens, necessitating methods for predicting meningococcal serogroup B (MenB) vaccine strain coverage. The genetic Meningococcal Antigen Typing System (gMATS), a correlate of MATS estimates, predicts strain coverage by the 4-component MenB (4CMenB) vaccine in cultivable and non-cultivable NmB isolates. In Taiwan, 134 invasive, disease-causing NmB isolates were collected in 2003-2020 (23.
View Article and Find Full Text PDFNat Commun
November 2023
Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Commissariat à l'Energie Atomique et aux Energies Alternatives, Université Grenoble Alpes; IRIG, CEA-Grenoble, 17 avenue des Martyrs, 38000, Grenoble, France.
Sanguina nivaloides is the main alga forming red snowfields in high mountains and Polar Regions. It is non-cultivable. Analysis of environmental samples by X-ray tomography, focused-ion-beam scanning-electron-microscopy, physicochemical and physiological characterization reveal adaptive traits accounting for algal capacity to reside in snow.
View Article and Find Full Text PDF3 Biotech
December 2023
Department of Marine Science, Bharathidasan University, Tiruchirappalli, 620 024 India.
Human pathogenic diseases received much attention recently due to their uncontrolled spread of antimicrobial resistance (AMR) which causes several threads every year. Effective alternate antimicrobials are urgently required to combat those disease causing infectious microbes. Halophilic actinobacteria revealed huge potentials and unexplored cultivable/non-cultivable actinobacterial species producing enormous antimicrobials have been proved in several genomics approaches.
View Article and Find Full Text PDFFront Cell Infect Microbiol
July 2023
Adaptation et Santé des Invertébrés Marins (ASIM), Ifremer, La Tremblade, France.
Introduction: and are protozoan parasites responsible for mortalities of farmed and wild flat oysters Ostrea edulis in Europe since 1968 and 1979, respectively. Despite almost 40 years of research, the life-cycle of these parasites is still poorly known, especially regarding their environmental distribution.
Methods: We carried out an integrated field study to investigate the dynamics of and in Rade of Brest, where both parasites are known to be present.
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