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Juvenile hormone mediates lipid storage in the oocytes of Dipetalogaster maxima. | LitMetric

Juvenile hormone mediates lipid storage in the oocytes of Dipetalogaster maxima.

Insect Biochem Mol Biol

Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina; Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Córdoba, Argentina. Electronic address:

Published: June 2021

AI Article Synopsis

  • - Triatomines, specifically "kissing bugs," are key vectors for Chagas disease and rely on blood meals to support their reproductive processes, primarily regulated by juvenile hormone (JH) during oogenesis and vitellogenesis.
  • - In studies using the species Dipetalogaster maxima, JH levels rise after feeding, boosting the expression of genes for yolk protein precursors and their receptors, which play roles in lipid and protein recruitment to the oocytes.
  • - Importantly, while JH regulates gene expression related to lipid storage in oocytes, the study found that lipid transfer isn't solely via the traditional Lp/LpR pathway, suggesting alternative mechanisms for lipid acquisition in these insects.

Article Abstract

Triatomines are vectors of Chagas disease and important model organisms in insect physiology. "Kissing bugs" are obligatory hematophagous insects. A blood meal is required to successfully complete oogenesis, a process primarily controlled by juvenile hormone (JH). We used Dipetalogaster maxima as an experimental model to further understand the roles of JH in the regulation of vitellogenesis and oogenesis. A particular focus was set on the role of JH controlling lipid and protein recruitment by the oocytes. The hemolymph titer of JH III skipped bisepoxide increased after a blood meal. Following a blood meal there were increased levels of mRNAs in the fat body for the yolk protein precursors, vitellogenin (Vg) and lipophorin (Lp), as well as of their protein products in the hemolymph; mRNAs of the Vg and Lp receptors (VgR and LpR) were concomitantly up-regulated in the ovaries. Topical administration of JH induced the expression of Lp/LpR and Vg/VgR genes, and prompted the uptake of Lp and Vg in pre-vitellogenic females. Knockdown of the expression of LpR by RNA interference in fed females did not impair the Lp-mediated lipid transfer to oocytes, suggesting that the bulk of lipid acquisition by oocytes occurred by other pathways rather than by the endocytic Lp/LpR pathway. In conclusion, our results strongly suggest that JH signaling is critical for lipid storage in oocytes, by regulating Vg and Lp gene expression in the fat body as well as by modulating the expression of LpR and VgR genes in ovaries.

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Source
http://dx.doi.org/10.1016/j.ibmb.2020.103499DOI Listing

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