AI Article Synopsis
- Taraxacum mongolicum is a traditional Chinese medicine known for its use in treating inflammatory and infectious diseases, including mastitis, but its mechanisms of action remain unclear.
- The study aimed to uncover the protective effects and mechanisms of T. mongolicum against mastitis caused by Staphylococcus aureus in mice.
- Results showed that T. mongolicum reduced inflammatory cytokines, improved tissue health, and inhibited specific signaling pathways linked to inflammation, suggesting its potential as a treatment for mastitis.
Article Abstract
Ethnopharmacological Relevance: As a traditional Chinese medicine, Taraxacum mongolicum has been widely used for the prevention and treatment of a variety of inflammatory and infectious diseases, and also clinically used as a remedy for mastitis. However, the scientific rationale and mechanism behind its use on mastitis in vivo are still unclear.
Aim Of The Study: This study aimed to investigate the protective effect and potential mechanism of Taraxacum mongolicum Hand.-Mazz. (T. mongolicum) on mastitis infected by Staphylococcus aureus (S. aureus).
Materials And Methods: Female ICR mice were given intragastrically 2.5, 5 and 10 g/kg of T. mongolicum extract twice per day for 6 consecutive days, and infected with S. aureus via teat canal to induce mastitis. Pro-inflammatory cytokine tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) levels were determined by ELISA. Myeloperoxidase (MPO) activity and distribution were measured by reagent kit and immunohistochemistry. Histopathological changes of mammary gland tissues were observed by H&E staining. Toll-like receptor 2 (TLR2) expression, phosphorylations of related proteins in nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) signaling pathways were detected by western blot.
Results: T. mongolicum decreased TNF-α, IL-6 and IL-1β levels, and reduced MPO activity and distribution in sera and mammary glands with S. aureus-infected mastitis. In addition, T. mongolicum effectively attenuated histopathological damages and cell necrosis of mammary gland tissues infected by S. aureus. Moreover, T. mongolicum inhibited the expression of TLR2, and the phosphorylations of inhibitor κBα (IκBα), p65, p38, extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun N-terminal kinase (JNK) proteins in mammary glands with S. aureus-infected mastitis.
Conclusions: This study suggests that T. mongolicum protects against S. aureus-infected mastitis by exerting anti-inflammatory role, which is attributed to the inhibition of TLR2-NF-κB/MAPKs pathways.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jep.2020.113595 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Taraxasterol regulates p53 transcriptional activity to inhibit pancreatic cancer by inducing MDM2 ubiquitination degradation.
Phytomedicine
December 2024
Key Laboratory Pathobiology, State Ethnic Affairs Commission, Yanbian University, Yanji 133000, PR China. Electronic address:
Background: Pancreatic cancer (PC) is a malignant tumor with complex development mechanisms and a poor prognosis. Taraxasterol (TAX), a pentacyclic triterpenoid plant sterol derived from Taraxacum mongolicum, has multiple biological activities including an anti-tumor effect. However, the mechanism by which TAX exerts its anticancer effects in PC remains unclear.
View Article and Find Full Text PDFTmCOP1-TmHY5 module-mediated blue light signal promotes chicoric acid biosynthesis in Taraxacum mongolicum.
Plant Biotechnol J
December 2024
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Gem, Sun Yat-Sen), Nanjing, China.
Chicoric acid, a phenolic compound derived from plants, exhibits a range of pharmacological activities. Light significantly influences the chicoric acid biosynthesis in Taraxacum mongolicum; however, the transcriptional regulatory network governing this process remains unclear. A combined analysis of the metabolome and transcriptome revealed that blue light markedly enhances chicoric acid accumulation compared to red light.
View Article and Find Full Text PDFTaraxacum Mongolicum Polysaccharides Reverses Mice Obesity via Activation of AKT/mTOR Pathway.
Nutrients
September 2024
State Key Laboratory of Animal Biotech Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Background/objectives: The global prevalence of obesity and its associated health complications represent significant public health concerns. Plant polysaccharides have been demonstrated to possess a range of beneficial pharmacological effects. This experiment was designed to elucidate the mechanisms of dietary Taraxacum mongolicum polysaccharides involved in the regulation of obesity and fat browning.
View Article and Find Full Text PDFInvestigation of the Lipid-Lowering Activity and Mechanism of Three Extracts from , L., and Hand. Mazz Based on Network Pharmacology and In Vitro and In Vivo Experiments.
Foods
September 2024
College of Life Sciences, Northeast Forestry University, Harbin 150040, China.
Article Synopsis
- Hyperlipidemia is a condition where there is an abnormal amount of lipids in the bloodstream, and this study explores how extracts from various medicinal and edible plants can help reduce it.
- The research utilizes advanced methods like LC-MS and network pharmacology to identify active compounds and targets related to hyperlipidemia, leading to the discovery of 41 compounds and 140 targets.
- Experimental results demonstrate that a specific combination of plant extracts (AMH:HRE:TME in a ratio of 3:1:2) effectively lowers lipid levels and enhances antioxidant properties, confirming that it influences key signaling pathways related to lipid metabolism.
Integrative analysis of the metabolome and transcriptome reveals the mechanism of polyphenol biosynthesis in .
Front Plant Sci
August 2024
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
Introduction: Dandelion is widely used in clinical practice due to its beneficial effects. Polyphenolic compounds are considered the main anti-inflammatory active ingredient of dandelion, but the gene expression patterns of polyphenolic compounds in different dandelion tissues are still unclear.
Methods: In this study, we combined a nontargeted metabolome, PacBio Iso-seq transcriptome, and Illumina RNA-seq transcriptome to investigate the relationship between polyphenols and gene expression in roots, flowers, and leaves of flowering dandelion plants.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!