A novel method for fabricating air-cathodes was developed by assembling an activated carbon (AC) catalyst together with a waterproof breathable membrane (WBM) and stainless steel mesh (SSM) to reduce manufacturing costs of bioelectrochemical systems (BESs). WBMs made of different materials were tested in the assembly, including a hybrid of polypropylene and polyolefin (PPPO), polyethylene (PE), and polyurethane (PU), and compared against poly tetrafluoroethylene (PTFE)-based cathodes. Results showed that the maximum power density of the activated carbon-stainless steel mesh-polyurethane (AC@SSM/PU) assembly was 2.03 W/m while that of conventional carbon cloth cathode assembly (Pt@CC/PTFE) was 1.51 W/m. Compared to conventional cathode fabrication, AC@SSM/PU had a much lower cost and simpler manufacturing process. Illumina Miseq sequencing of 16S rRNA gene amplicons indicated that microbiomes were substantially different between anode and cathode biofilms. There was also a difference in the community composition between different cathode biofilms. The predominant population in the anode biofilms was Geobacter (38-75% relative abundance), while Thauera and Pseudomonas dominated the cathode biofilms. The results demonstrated that different types of air-cathodes influenced the microbial community assembly on the electrodes.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142281 | DOI Listing |
Environ Res
January 2025
School of Environmental Science and Engineering, Guangzhou University, Guangzhou, 510006, PR. China; Guangzhou University-Linköping University Research Center on Urban Sustainable Development, Guangzhou University, Guangzhou 510006, PR. China. Electronic address:
Antimony (Sb) contamination in water resources poses a critical environmental and health challenge globally. Sulfate reducing bacteria (SRB) are employed to reduce SO to S for removing Sb in a microbial electrolysis cell (MEC). Yet, the reduction efficiency of reducing SO and Sb(Ⅴ) through SRB remains relatively low, and the underlying mechanism remains elusive.
View Article and Find Full Text PDFSci Rep
January 2025
Nottingham Ningbo China Beacons of Excellence Research and Innovation Institute, University of Nottingham Ningbo China, Ningbo, China.
Electrodes functionalised with weak electroactive microorganisms offer a viable alternative to conventional chemical sensors for detecting priority pollutants in bioremediation processes. Biofilm-based biosensors have been proposed for this purpose. However, biofilm formation and maturation require 24-48 h, and the microstructure and coverage of the electrode surface cannot be controlled, leading to poorly reproducible signal and sensitivity.
View Article and Find Full Text PDFFront Microbiol
January 2025
School of Engineering, Newcastle University, Newcastle upon Tyne, United Kingdom.
Microbial Fuel Cells (MFCs) are innovative environmental engineering systems that harness the metabolic activities of microbial communities to convert chemical energy in waste into electrical energy. However, MFC performance optimization remains challenging due to limited understanding of microbial metabolic mechanisms, particularly with complex substrates under realistic environmental conditions. This study investigated the effects of substrate complexity (acetate vs.
View Article and Find Full Text PDFJ Environ Manage
February 2025
Department of Chemical & Biomolecular Engineering, Institute for Corrosion and Multiphase Technology, Ohio University, Athens, 45701, USA; Department of Biological Sciences, Molecular & Cellular Biology Program, Ohio University, Athens, OH, 45701, USA. Electronic address:
Biofilms can cause biofouling, water quality deterioration, and transmission of infectious diseases. They are also responsible for microbiologically influenced corrosion (MIC) which can cause leaks, resulting in environmental disasters. A new disposable biofilm/MIC test kit was demonstrated to distinguish abiotic corrosion of carbon steel from MIC.
View Article and Find Full Text PDFSensors (Basel)
December 2024
Department of Physics, Yonsei University, Seoul 03722, Republic of Korea.
The rapid and reliable detection of pathogenic bacteria remains a significant challenge in clinical microbiology. Consequently, the demand for simple and rapid techniques, such as antimicrobial peptide (AMP)-based sensors, has recently increased as an alternative to traditional methods. Melittin, a broad-spectrum AMP, rapidly associates with the cell membranes of various gram-positive and gram-negative bacteria.
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