Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
Brucellosis is a severe public health problem in the Inner Mongolia regions of China. The recent prevalence of brucellosis outbreaks may be attributed to an increase in the activity of ticks and other air-borne vectors. () is a native tick species of Inner Mongolia; similar to other tick species, carries a variety of pathogens that can be transmitted to a wide range of animals. In this study, we have investigated the potential of in transmitting brucellosis. From 2015 to 2019, 2,256 ticks were collected from 23 different pastoral areas of Hulun Buir. Brucellosis pathogen was detected using DNA extracted from different developmental stages of ticks. Salivary gland and midgut tissue samples were used as templates to amplify gene ( genus-specific gene) by using TaqMan Real-Time polymerase chain reaction (PCR). To detect the presence of Bscp31 protein, which is specific to spp., in the midgut and salivary gland tissues of , Western blotting and immunofluorescence were performed. Additionally, spp. were isolated using a culture medium. Tick samples were identified as . Different percentages of genus-specific genes could be found in the tick samples. From 2015 to 2019, the positivity rate for the detection of gene in ranged from 0.00 to 87.80%, with the highest rate of 89.00%. In addition, genus-specific genes were successfully detected in the samples isolated from all the developmental stages and anatomical regions of ticks. Bscp31 protein was present in the midgut and salivary gland of . Further, biotype 3 was isolated from eggs and engorged adults of . These findings demonstrate that is a potent, long-term carrier of spp. that can exhibit transovarial transmission potential, presenting as a novel arthropod host for spp. This study, therefore, indicates the potential risk of transmission of brucellosis via tick bites among animals as well as human beings.
Download full-text PDF |
Source |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7649779 | PMC |
http://dx.doi.org/10.3389/fvets.2020.566253 | DOI Listing |
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