Peculiar Hosts Fail to Establish a Stable Intracellular Relationship With .

, an intracellular human pathogen, establishes intracellular relationships with several protist hosts, including . can escape the normal digestion process and establish intracellular relationships in . In this study, we identify new strains that significantly reduce the number of during infection. As a result, stable intracellular relationships between and these strains were not observed. These digestion-type also showed high efficiency for elimination compared to other strains of . These results suggest that the digestion-type strains identified have high non-specific digestion activity. Although we evaluated the maturation process of -containing vacuoles (LCVs) in the strains using LysoTracker, there were no discriminative changes in these LCVs compared to other strains. Detailed understanding of the mechanisms of high digestion efficiency in these strains could be applied to water purification technologies and elimination from environmental water.