AI Article Synopsis
- MicroRNAs, specifically miR-100 and miR-210, are important for diagnosing and understanding the prognosis of pediatric acute lymphoblastic leukemia (ALL).
- In patients, miR-100 is significantly lower and miR-210 higher compared to healthy controls, indicating their potential as markers; miR-100 also correlates with poorer outcomes.
- While miR-100 proves to be a useful prognostic biomarker related to survival rates, miR-210 did not show significant associations with patient outcomes.
Article Abstract
Background: : microRNAs are playing important roles in the diagnosis and prognosis of pediatric acute lymphoblastic leukemia (ALL).
Methods: Expression levels of miR-100 and miR-210 were assessed in bone marrow aspirate of 85 pediatric ALL patients compared to 12 healthy control using quantitative real-time polymerase chain reaction. Data were correlated with relevant clinico-pathological features of the patients, response to treatment, disease-free survival (DFS), and overall survival (OS).
Results: miR-100 was significantly downregulated in ALL patients [median: 1.21, range: 0-434.3] compared to the control group [median: 8.41, range; 0-840.3, = 0.035]. miR-210 was significantly upregulated in ALL patients [median: 6.34, range: 1.16-1088.7] compared to the control group [median: 2.57, range: 0.11-709.2, = 0.025]. The sensitivity, specificity, and area under curve of miR-100 were (64.7%, 62.5%, and 0.642; respectively, = 0.035) at a cut-off 2.6 and that of miR-210 were (60%, 58.3% and 0.650; respectively, = 0.025) at a cut-off 3.5. miR-100 overexpression associated with shorter DFS and OS (= 0.033 and 0.046; respectively). Patients with miR-100 lowexpression showed a significant incidence of late death = 0.024. There was no significant association between miR-210 expression and DFS, OS, incidence of early or late death.
Conclusion: : miR-100 and miR-210 could be used as potential diagnostic markers for pediatric ALL. miR-100 is a useful prognostic and predictive biomarker for childhood ALL.
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| http://dx.doi.org/10.1080/16078454.2020.1843753 | DOI Listing |
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