The feasibility of sugarcane bagasse to be employed as an alternative solid support for clean-up is presented during the development and validation of an analytical method to determine chlorpyrifos (O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphorothioate) (CP) in tomato by HPLC-DAD. The efficiency of the method consisting of ultrasonic assisted extraction with acetonitrile and dispersion with salts, followed by dispersive phase extraction with powdered sugarcane bagasse is presented for three spiking levels relating to the established CP maximum residue limit (MRL) for industrial tomato in Brazil, 0.5 mg kg (½ MRL, MRL and 2 MRL). Satisfactory results were obtained in terms of trueness (recovery range: 76.5-120.1%), intermediate precision (1.0-5.2%) and repeatability (4.7-12.8%), expressed as relative standard deviation. The proposed method has a limit of quantification of 0.25 mg kg and could be deployed as cost effective routine analysis to monitor CP in tomato samples in processed food industries.
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