Hydrogenation of carbon dioxide (CO) to formic acid by the enzyme formate dehydrogenase (FDH) is a promising technology for reducing CO concentrations in an environmentally friendly manner. However, the easy separation of FDH with enhanced stability and reusability is essential to the practical and economical implementation of the process. To achieve this, the enzyme must be used in an immobilized form. However, conventional immobilization by physical adsorption is prone to leaching, resulting in low stability. Although other immobilization methods (such as chemical adsorption) enhance stability, they generally result in low activity. In addition, mass transfer limitations are a major problem with most conventional immobilized enzymes. In this review paper, the effectiveness of metal organic frameworks (MOFs) is assessed as a promising alternative support for FDH immobilization. Kinetic mechanisms and stability of wild FDH from various sources were assessed and compared to those of cloned and genetically modified FDH. Various techniques for the synthesis of MOFs and different immobilization strategies are presented, with special emphasis on in situ and post synthetic immobilization of FDH in MOFs for CO hydrogenation.
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