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Macrophage Activation Assays to Evaluate the Immunostimulatory Capacity of in A Multivalent Poultry Vaccine. | LitMetric

Macrophage Activation Assays to Evaluate the Immunostimulatory Capacity of in A Multivalent Poultry Vaccine.

Vaccines (Basel)

Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584CL Utrecht, The Netherlands.

Published: November 2020

AI Article Synopsis

  • * The study uses a chicken macrophage-like cell line, HD11, to evaluate the immune response to an inactivated multivalent vaccine targeting multiple poultry diseases, showing it stimulates nitric oxide production and pro-inflammatory cytokine expression.
  • * The findings suggest that lipopolysaccharides are important for these immune responses and indicate that this vaccine can effectively activate immune cells, paving the way for in vitro quality testing of vaccines in the future.

Article Abstract

High-quality vaccines are crucial to prevent infectious disease outbreaks in the poultry industry. In vivo vaccination tests are routinely used to test poultry vaccines for their potency, i.e., their capacity to induce protection against the targeted diseases. A better understanding of how poultry vaccines activate immune cells will facilitate the replacement of in vivo potency tests for in vitro assays. Using the chicken macrophage-like HD11 cell line as a model to evaluate innate immune responses, the current explorative study addresses the immunostimulatory capacity of an inactivated multivalent vaccine for infectious bronchitis, Newcastle disease, egg-drop syndrome, and infectious coryza. The vaccine stimulated HD11 cells to produce nitric oxide and to express pro-inflammatory cytokines , and chemokines and , and the anti-inflammatory cytokine , but only when inactivated , the causative agent of infectious coryza, was present. Lipopolysaccharides from were crucial for the production of nitric oxide and expression of and . The described immune parameters demonstrate the capacity of this multivalent vaccine to activate innate immune cells and may in the future, combined with antigen quantification methods, contribute to vaccine quality testing in vitro, hence the replacement of current in vivo vaccination tests.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712920PMC
http://dx.doi.org/10.3390/vaccines8040671DOI Listing

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