Improved green biosynthesis of chitosan decorated Ag- and CoO-nanoparticles: A relationship between surface morphology, photocatalytic and biomedical applications.

AgNPs@Chitosan and CoO-NPs@Chitosan were fabricated with Salvia hispanica. Results showed MZI values of 5 and 30 mm for CoO-NPs- and AgNPs@Chitosan against S. aureus, and 15 and 21 mm for CoO-NPs- and AgNPs@Chitosan against E. coli (24 h, 20 μg/mL), respectively. MTT assays showed up to 80% and 90%, 71% and 75%, and 91% and 94% mammalian cell viability for the green synthesized, chemically synthesized AgNPs and green synthesized AgNPs@Chitosan for HEK-293 and PC12 cells, respectively, and 70% and 71%, 59% and 62%, and 88% and 73% for the related CoO-NPs (24 h, 20 μg/mL). The photocatalytic activities showed dye degradation after 135 and 105 min for AgNPs@Chitosan and CoO-NPs@Chitosan, respectively. FESEM results showed differences in particle sizes (32 ± 3.0 nm for the AgNPs and 41 ± 3.0 nm for the CoONPs) but AFM results showed lower roughness of the AgNPs@Chitosan (7.639 ± 0.85 nm) compared to CoONPs@Chitosan (9.218 ± 0.93 nm), which resulted in potential biomedical applications.