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Combination of Probe Electrospray Ionization Mass Spectrometry and Mass Spectrometry Imaging to Analyze Plant Alkaloids in .
Mass Spectrom (Tokyo)
December 2024
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Plant alkaloids are used in a variety of medicines, such as anti-cancer drugs and analgesics. Among these plant alkaloids, galanthamine is an Amaryllidaceae type alkaloid with acetylcholinesterase inhibition activity used in the treatment of neurological diseases such as Alzheimer's disease. Although the chemical synthesis of galanthamine has been successfully achieved, is the main source of its production.
View Article and Find Full Text PDFChemical and metabolic profiling of Amaryllidaceae alkaloids in the bulbs of Narcissus tazetta L. var. chinensis Roem using liquid chromatography-tandem mass spectrometry with data mining strategy.
Rapid Commun Mass Spectrom
January 2025
Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang, China.
Rationale: The bulbs of Narcissus tazetta L. var. chinensis Roem (BNTCR) has been used as an herbal medicine for the treatment of carbuncles in China.
View Article and Find Full Text PDFDiurnal patterns of floral volatile emissions in three species of Narcissus.
Am J Bot
October 2024
Bonner Institut für Organismische Biologie (BIOB) Dept. Biodiversity of Plants, Mathematisch-Naturwissenschaftliche Fakultät, Rheinische Friedrich-Wilhelmsuniversität Bonn, Bonn, 53115, Germany.
Premise: Plants generate a wide array of signals such as olfactory cues to attract and manipulate the response of pollinators. The present study addresses the temporal patterns of scent emission as an additional dimension to the scent composition. The expectation is that divergent floral function is reflected in divergent qualitative and temporal emission patterns.
View Article and Find Full Text PDFWe have developed a rapid genus identification method for poisonous plants. The real-time PCR using the TaqMan probe method was employed for detection, with the amplified targets being the "trnL (UAA)-intron" or "trnL-trnF intergenic spacer" regions of chloroplast DNA. The targeted plants were selected six genera (Aconitum, Colchicum, Veratrum, Brugmansia, Scopolia and Narcissus), which have been implicated in many instances of food poisoning in Japan.
View Article and Find Full Text PDFComparative Analysis of Primary and Secondary Metabolites in Different In Vitro Tissues of var. chinensis.
ACS Omega
June 2024
Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea.
Article Synopsis
- The study focuses on var. chinensis, a plant known for its pharmaceutical and ornamental uses, by analyzing primary and secondary metabolites in different in vitro tissues (callus, adventitious roots, and shoots) using advanced chromatography techniques.
- The researchers optimized in vitro culture methods using hormonal treatments on bulbs to promote growth and metabolite production, with specific concentrations found to be most effective.
- Results indicated that shoots and roots cultivated in a particular nutrient medium yielded the highest levels of metabolites, suggesting that this method could be beneficial for mass-producing valuable compounds from the plant.
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